The CD105:CD106 microparticle ratio is CD106 dominant in polycystic ovary syndrome compared to type 2 diabetes and healthy subjects

• Published in: Endocrine Vol. 66; no. 2; pp. 220 - 225
• Main Authors: Al-Qaissi, Ahmed, Alqarni, Saeed, Javed, Zeeshan, Atkin, Stephen L., Sathyapalan, Thozhukat, Vince, Rebecca V., Madden, Leigh A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2019; Springer Nature B.V
• Subjects: Adult; Biomarkers - blood; CD105 antigen; Cell adhesion & migration; Cell adhesion molecules; Cell-Derived Microparticles - metabolism; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - metabolism; Endocrinology; Endoglin; Endoglin - metabolism; Female; Flow cytometry; Humanities and Social Sciences; Humans; Intercellular adhesion molecule 1; Intercellular Adhesion Molecule-1 - blood; Internal Medicine; Medicine; Medicine & Public Health; Microparticles; multidisciplinary; Original; Original Article; Polycystic ovary syndrome; Polycystic Ovary Syndrome - metabolism; Retrospective Studies; Risk Factors; Science; Vascular cell adhesion molecule 1; Vascular Cell Adhesion Molecule-1 - metabolism; Young Adult; Polycystic ovary syndrome; VCAM-1; Endothelial microparticles
• ISSN: 1355-008X; 1559-0100; 1559-0100
• DOI: 10.1007/s12020-019-02059-9

Background A retrospective analysis was carried out from patients and controls during the past 5 years from a series of studies investigating endothelial microparticles (MP). Methods In total, 319 samples from 207 individuals were included in this analysis, from patients with type 2 diabetes (T2D, n  = 105), women with polycystic ovary syndrome (PCOS, n  = 145) and healthy volunteers ( n  = 69). All data were generated via the same flow cytometry protocol with the same antibody clones. Endothelial markers CD105 (Endoglin) and CD106 (Vascular cell adhesion molecule-1) were used to enumerate MP in venous blood. Results The ratio of CD105MP:CD106MP was significantly different between groups ( F  = 63.43, p  < 0.0001). Women with PCOS were found to have a median CD105MP:CD106MP ratio of 0.40 (IQR 0.24–0.57), suggesting approximately two CD106MP were found per CD105MP. The T2D group showed a median ratio of 2.32 (1.51–3.69) whereas in healthy volunteers the ratio was 2.21 (1.63–3.55). Serum intercellular adhesion molecule-1 was also shown to be significantly increased in PCOS when compared with control or T2D groups ( F  = 14.5, p  < 0.001). Conclusion These data suggest that women with PCOS have an altered endothelial MP release in favour of CD106. Thus a potential activated endothelial state exists in women with PCOS with a shift towards a predominantly CD106MP profile.