Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)
Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colo...
Saved in:
| Published in | Water research (Oxford) Vol. 157; pp. 201 - 208 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
15.06.2019
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research.
EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS.
This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality.
[Display omitted]
•Colorimetric EPS analysis is very sensitive towards the standard selection.•Analysis of single EPS compounds is prone to interference by other EPS compounds.•EPS quantification with colorimetric methods can easily give inaccurate results.•New approaches and methods are needed to accurately characterize EPS. |
|---|---|
| AbstractList | Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research.
EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS.
This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality.
[Display omitted]
•Colorimetric EPS analysis is very sensitive towards the standard selection.•Analysis of single EPS compounds is prone to interference by other EPS compounds.•EPS quantification with colorimetric methods can easily give inaccurate results.•New approaches and methods are needed to accurately characterize EPS. Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS. This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality. |
| Author | Felz, Simon Vermeulen, Pascalle van Loosdrecht, Mark C.M. Lin, Yue Mei |
| Author_xml | – sequence: 1 givenname: Simon surname: Felz fullname: Felz, Simon email: s.felz@tudelft.nl – sequence: 2 givenname: Pascalle surname: Vermeulen fullname: Vermeulen, Pascalle – sequence: 3 givenname: Mark C.M. orcidid: 0000-0003-0658-4775 surname: van Loosdrecht fullname: van Loosdrecht, Mark C.M. – sequence: 4 givenname: Yue Mei surname: Lin fullname: Lin, Yue Mei |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30953855$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9kE1v1DAQhi1URLeFf4CQj-WQMLGdOLkgoVVbkCqBBJwtf0y0XiXxYjuF5dfj1RaOnObyPu_MPFfkYgkLEvK6gbqBpnu3r3_qHDHVDJqhBl5D1z8jm6aXQ8WE6C_IBkDwquGtuCRXKe0BgDE-vCCXHIaW9227IX67w9lbPVG701HbjNH_1tmHhc6Yd8ElOoZI8w6pXvR0TD7RMNKU42rzGguHv3LhcJrWSUd6CNNxLh2WptWkrBeLid7cfvn69iV5Puop4auneU2-391-236sHj7ff9p-eKiskCJXkiNq13dGOz0MHWdopAVoHRfcCMs4GGmsaIWUvXXGuLaXAgY3GjM611t-TW7OvYcYfqyYspp9Ot2nFwxrUoyB6AYmgZeoOEdtDClFHNUh-lnHo2pAnSSrvTpLVifJCrgqkgv25mnDamZ0_6C_Vkvg_TmA5c9Hj1El67GocD6izcoF__8NfwDO1pSC |
| CitedBy_id | crossref_primary_10_1016_j_jksus_2023_102688 crossref_primary_10_1016_j_wroa_2024_100216 crossref_primary_10_1016_j_soilbio_2020_107961 crossref_primary_10_2139_ssrn_3999121 crossref_primary_10_1016_j_foodres_2022_112094 crossref_primary_10_1016_j_scitotenv_2021_149907 crossref_primary_10_1016_j_jece_2020_104156 crossref_primary_10_3390_en14217219 crossref_primary_10_1007_s00449_020_02439_w crossref_primary_10_1016_j_envres_2023_117709 crossref_primary_10_1016_j_seppur_2022_120787 crossref_primary_10_1016_j_watres_2019_114952 crossref_primary_10_1016_j_ijbiomac_2023_127625 crossref_primary_10_1128_aem_01928_22 crossref_primary_10_1016_j_ijbiomac_2024_130731 crossref_primary_10_1016_j_chemosphere_2020_128033 crossref_primary_10_1016_j_jwpe_2022_103076 crossref_primary_10_1039_D3RA06803J crossref_primary_10_1016_j_watres_2022_119041 crossref_primary_10_1016_j_jwpe_2023_104540 crossref_primary_10_1016_j_scitotenv_2022_160442 crossref_primary_10_1016_j_watres_2024_121240 crossref_primary_10_1016_j_watres_2020_115924 crossref_primary_10_2166_wst_2022_089 crossref_primary_10_1007_s11356_022_21645_5 crossref_primary_10_1016_j_lwt_2021_112958 crossref_primary_10_1016_j_watres_2022_119395 crossref_primary_10_1016_j_watres_2020_115833 crossref_primary_10_3389_fmicb_2022_982745 crossref_primary_10_1016_j_resconrec_2022_106306 crossref_primary_10_4103_pm_pm_42_21 crossref_primary_10_1016_j_scitotenv_2023_164177 crossref_primary_10_1016_j_memsci_2020_118835 crossref_primary_10_1016_j_bcdf_2021_100297 crossref_primary_10_1016_j_watres_2023_119687 crossref_primary_10_1016_j_biortech_2021_125167 crossref_primary_10_1016_j_chemosphere_2024_141170 crossref_primary_10_1016_j_watres_2019_115344 crossref_primary_10_1080_09593330_2024_2338456 crossref_primary_10_1007_s13762_021_03466_0 crossref_primary_10_1021_acs_estlett_0c00784 crossref_primary_10_1016_j_ijbiomac_2024_129954 crossref_primary_10_1016_j_chemosphere_2020_126107 crossref_primary_10_1016_j_scitotenv_2022_155673 crossref_primary_10_1016_j_biortech_2021_125255 crossref_primary_10_1016_j_biortech_2023_129618 crossref_primary_10_1016_j_jwpe_2024_104994 crossref_primary_10_1021_acsestwater_4c00217 crossref_primary_10_1016_j_carres_2023_108962 crossref_primary_10_1007_s10123_023_00366_w crossref_primary_10_1016_j_cej_2022_137844 crossref_primary_10_1080_09593330_2022_2159541 crossref_primary_10_2166_ws_2023_078 crossref_primary_10_1016_j_biortech_2021_126592 crossref_primary_10_2139_ssrn_3961582 crossref_primary_10_3390_separations8080120 crossref_primary_10_3390_membranes12020210 crossref_primary_10_1016_j_coesh_2019_09_011 crossref_primary_10_1016_j_jwpe_2023_104346 crossref_primary_10_1007_s11814_023_1407_1 crossref_primary_10_1016_j_cej_2022_135792 crossref_primary_10_14202_vetworld_2021_847_853 crossref_primary_10_1016_j_biortech_2020_124554 crossref_primary_10_1016_j_watres_2019_115291 crossref_primary_10_1016_j_micres_2021_126877 crossref_primary_10_1016_j_ijbiomac_2024_129952 crossref_primary_10_2166_wst_2024_158 crossref_primary_10_1016_j_corsci_2022_110703 crossref_primary_10_1016_j_watres_2024_121512 crossref_primary_10_3390_membranes13010074 crossref_primary_10_1016_j_chemosphere_2020_127539 crossref_primary_10_1016_j_jenvman_2022_116247 crossref_primary_10_3390_app131910796 crossref_primary_10_1021_acs_est_0c00850 crossref_primary_10_1016_j_biortech_2022_127854 crossref_primary_10_1021_acsestwater_4c00118 crossref_primary_10_1016_j_jhazmat_2023_132146 crossref_primary_10_1016_j_corsci_2024_111985 crossref_primary_10_1002_elsc_202100076 crossref_primary_10_1016_j_carbpol_2021_118913 crossref_primary_10_1016_j_cclet_2021_08_002 crossref_primary_10_1016_j_scitotenv_2021_150434 crossref_primary_10_1016_j_jhazmat_2019_121782 crossref_primary_10_1186_s12934_023_02240_w crossref_primary_10_1016_j_cej_2024_150528 crossref_primary_10_1007_s00253_023_12465_9 crossref_primary_10_1016_j_watres_2023_119800 crossref_primary_10_1016_j_biortech_2021_125482 crossref_primary_10_1021_acs_est_1c05289 crossref_primary_10_1016_j_biochi_2024_03_006 crossref_primary_10_1016_j_jece_2021_106563 crossref_primary_10_1016_j_biortech_2022_126747 crossref_primary_10_1021_acssynbio_9b00192 crossref_primary_10_1016_j_watres_2021_117706 crossref_primary_10_13005_ojc_390619 crossref_primary_10_1016_j_jece_2024_112178 crossref_primary_10_1007_s11356_023_31071_w crossref_primary_10_1016_j_procbio_2021_06_022 crossref_primary_10_2139_ssrn_4180193 crossref_primary_10_1016_j_chemosphere_2024_141778 crossref_primary_10_1007_s12649_020_01252_6 crossref_primary_10_1111_jam_15455 crossref_primary_10_3390_ijms242115904 crossref_primary_10_1007_s11756_023_01580_7 crossref_primary_10_1016_j_foodres_2022_111739 crossref_primary_10_1016_j_chemosphere_2024_142402 crossref_primary_10_1016_j_biortech_2022_127343 crossref_primary_10_1016_j_enconman_2023_116747 crossref_primary_10_1021_acsomega_3c08475 crossref_primary_10_1016_j_jwpe_2022_103475 crossref_primary_10_2139_ssrn_3993318 crossref_primary_10_1016_j_cej_2024_152593 crossref_primary_10_1016_j_cej_2024_149074 crossref_primary_10_1007_s11356_020_07614_w crossref_primary_10_3923_pjbs_2021_1162_1168 crossref_primary_10_1016_j_scitotenv_2022_161371 crossref_primary_10_1016_j_biortech_2023_129273 crossref_primary_10_1016_j_biortech_2023_129155 crossref_primary_10_1016_j_jece_2019_103541 crossref_primary_10_1016_j_memsci_2022_121017 crossref_primary_10_1016_j_watres_2024_121438 crossref_primary_10_1021_acs_est_0c07442 crossref_primary_10_1016_j_scitotenv_2023_165549 crossref_primary_10_1016_j_biortech_2022_127804 crossref_primary_10_1016_j_envpol_2021_118470 crossref_primary_10_1016_j_lwt_2020_110210 crossref_primary_10_1016_j_ese_2024_100393 crossref_primary_10_1007_s11356_023_31755_3 crossref_primary_10_1016_j_cej_2023_144661 crossref_primary_10_1016_j_watres_2023_120275 crossref_primary_10_1016_j_biortech_2023_129968 crossref_primary_10_1016_j_scitotenv_2022_154472 crossref_primary_10_1016_j_biortech_2020_123632 crossref_primary_10_1021_acs_est_3c09302 crossref_primary_10_1016_j_bcab_2023_102909 crossref_primary_10_1016_j_ecoenv_2020_110441 crossref_primary_10_1016_j_jpha_2023_11_013 crossref_primary_10_1016_j_jwpe_2020_101148 crossref_primary_10_1016_j_jwpe_2024_105627 crossref_primary_10_1016_j_scitotenv_2020_142051 crossref_primary_10_1007_s00253_022_11866_6 crossref_primary_10_1016_j_watres_2023_120031 crossref_primary_10_1016_j_carbpol_2022_119536 crossref_primary_10_1016_j_jhazmat_2022_128361 crossref_primary_10_1016_j_scitotenv_2022_153657 crossref_primary_10_1016_j_lwt_2022_113860 crossref_primary_10_1016_j_biortech_2024_130966 crossref_primary_10_1016_j_memsci_2020_118189 crossref_primary_10_1016_j_corsci_2023_111485 crossref_primary_10_1016_j_cej_2023_145628 crossref_primary_10_1007_s42114_022_00508_0 crossref_primary_10_1016_j_scitotenv_2023_168877 crossref_primary_10_1016_j_micres_2024_127674 crossref_primary_10_1016_j_bej_2022_108500 crossref_primary_10_1016_j_chemosphere_2021_130170 crossref_primary_10_1016_j_jenvman_2020_110394 crossref_primary_10_3390_ijerph18189466 crossref_primary_10_1016_j_jenvman_2021_112696 crossref_primary_10_1002_est2_634 crossref_primary_10_3389_fmicb_2021_656380 crossref_primary_10_1016_j_watres_2020_115617 crossref_primary_10_1007_s00216_020_02955_4 crossref_primary_10_1016_j_chemosphere_2022_134935 crossref_primary_10_3390_md21010036 crossref_primary_10_1016_j_fuel_2023_129158 crossref_primary_10_2147_IJN_S323685 crossref_primary_10_3390_microorganisms9061185 crossref_primary_10_1016_j_watres_2023_120985 crossref_primary_10_1016_j_eti_2020_101053 crossref_primary_10_1016_j_jhazmat_2022_128797 crossref_primary_10_1016_j_microc_2020_104831 crossref_primary_10_1016_j_biortech_2020_123608 crossref_primary_10_1021_acsestengg_1c00257 crossref_primary_10_1016_j_jhazmat_2020_123506 crossref_primary_10_1360_TB_2022_0636 crossref_primary_10_1016_j_colsurfb_2020_111160 crossref_primary_10_1016_j_jhazmat_2024_134244 crossref_primary_10_1007_s00253_020_10751_4 crossref_primary_10_2139_ssrn_3996844 crossref_primary_10_1016_j_scitotenv_2021_150730 crossref_primary_10_1016_j_envres_2023_117613 crossref_primary_10_1016_j_jclepro_2024_141668 crossref_primary_10_1021_acsearthspacechem_0c00252 crossref_primary_10_1016_j_mtbio_2024_101091 crossref_primary_10_3389_fmicb_2022_963260 crossref_primary_10_1016_j_cej_2024_149819 crossref_primary_10_1016_j_watres_2020_116391 crossref_primary_10_1016_j_scitotenv_2020_144798 crossref_primary_10_1016_j_scitotenv_2023_164246 crossref_primary_10_1016_j_biortech_2024_131054 crossref_primary_10_1016_j_jenvman_2020_111834 crossref_primary_10_1016_j_bioflm_2019_100011 crossref_primary_10_1039_D3EW00050H |
| Cites_doi | 10.1021/jf0502698 10.1016/j.watres.2007.11.011 10.1016/S0021-9258(18)84277-6 10.1016/S0021-9258(19)51173-5 10.1016/j.carres.2007.03.031 10.1016/0003-2697(91)90372-Z 10.1126/science.1058890 10.1016/j.scitotenv.2010.05.039 10.1007/s00018-015-2004-1 10.1016/0003-2697(67)90142-X 10.1016/0003-2697(88)90383-1 10.3390/md8051567 10.1016/j.watres.2017.05.068 10.1021/jf1005935 10.1021/acs.est.5b05261 10.1038/nrmicro2415 10.1038/nchembio.437 10.1016/0003-2697(85)90442-7 10.1021/i560156a015 10.1016/j.watres.2016.03.008 10.1016/S0021-9258(17)35155-4 10.2136/sssaj2013.07.0287dgs 10.1016/0043-1354(83)90111-2 10.1016/0043-1354(95)00323-1 10.1111/j.1574-6968.2001.tb10937.x 10.1016/S0021-9258(19)52451-6 10.1016/j.biotechadv.2011.05.006 10.1021/jf021099r 10.1016/0003-2697(66)90050-9 10.1021/ac60111a017 10.1007/s002530050481 10.1016/j.watres.2013.03.010 |
| ContentType | Journal Article |
| Copyright | 2019 The Authors Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved. |
| Copyright_xml | – notice: 2019 The Authors – notice: Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved. |
| DBID | 6I. AAFTH NPM AAYXX CITATION 7X8 |
| DOI | 10.1016/j.watres.2019.03.068 |
| DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
| DatabaseTitleList | PubMed |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 1879-2448 |
| EndPage | 208 |
| ExternalDocumentID | 10_1016_j_watres_2019_03_068 30953855 S004313541930209X |
| Genre | Journal Article |
| GroupedDBID | --- --K --M -DZ -~X .DC .~1 0R~ 123 1B1 1RT 1~. 1~5 4.4 457 4G. 53G 5VS 6I. 7-5 71M 8P~ 9JM 9JN AABNK AACTN AAEDT AAEDW AAFTH AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAXUO ABFNM ABFRF ABFYP ABJNI ABLST ABMAC ABQEM ABQYD ABYKQ ACDAQ ACGFO ACGFS ACLVX ACRLP ACSBN ADBBV ADEZE AEBSH AEFWE AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGUBO AGYEJ AHEUO AHHHB AIEXJ AIKHN AITUG AJOXV AKIFW ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ATOGT AXJTR BKOJK BLECG BLXMC CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q GBLVA HMC IHE IMUCA J1W KCYFY KOM LY3 LY9 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 RIG ROL RPZ SCU SDF SDG SDP SES SPC SPCBC SSE SSJ SSZ T5K TAE TN5 TWZ WH7 XPP ZCA ZMT ~02 ~G- ~KM AAHBH AAXKI AKRWK NPM .55 186 29R 6TJ AAQXK AAYXX ABEFU ABTAH ABXDB ACKIV ADMUD AFFNX AFJKZ ASPBG AVWKF AZFZN CITATION FEDTE FGOYB G-2 HMA HVGLF HZ~ H~9 MVM OHT R2- SEN SEP SEW WUQ X7M XOL YHZ YV5 ZXP ZY4 ~A~ 7X8 |
| ID | FETCH-LOGICAL-c474t-73eead86bada99632eb7c005d343b4c230b7bc454778cdbbd587409dfbbfdd8c3 |
| IEDL.DBID | .~1 |
| ISSN | 0043-1354 |
| IngestDate | Fri Oct 25 04:09:38 EDT 2024 Thu Sep 26 15:28:59 EDT 2024 Wed Oct 16 00:49:47 EDT 2024 Fri Feb 23 02:23:34 EST 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | Colorimetric methods Biofilm Aerobic granular sludge Extracellular polymeric substances (EPS) EPS analysis |
| Language | English |
| License | This is an open access article under the CC BY-NC-ND license. Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c474t-73eead86bada99632eb7c005d343b4c230b7bc454778cdbbd587409dfbbfdd8c3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-0658-4775 |
| OpenAccessLink | https://www.sciencedirect.com/science/article/pii/S004313541930209X |
| PMID | 30953855 |
| PQID | 2204692703 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | proquest_miscellaneous_2204692703 crossref_primary_10_1016_j_watres_2019_03_068 pubmed_primary_30953855 elsevier_sciencedirect_doi_10_1016_j_watres_2019_03_068 |
| PublicationCentury | 2000 |
| PublicationDate | 2019-06-15 |
| PublicationDateYYYYMMDD | 2019-06-15 |
| PublicationDate_xml | – month: 06 year: 2019 text: 2019-06-15 day: 15 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Water research (Oxford) |
| PublicationTitleAlternate | Water Res |
| PublicationYear | 2019 |
| Publisher | Elsevier Ltd |
| Publisher_xml | – name: Elsevier Ltd |
| References | Avella, Görner, de Donato (bib2) 2010; 408 Flemming, Wingender (bib13) 2010; 8 Garza, Dutilh (bib18) 2015; 72 Smith, Krohn, Hermanson, Mallia, Gartner, Provenzano, Fujimoto, Goeke, Olson, Klenk (bib34) 1985; 150 Folin, Ciocalteau (bib14) 1927; 73 Sharma, Krishnan (bib33) 1966; 14 Galambos (bib17) 1967; 19 Ikawa, Schaper, Dollard, Sasner (bib19) 2003; 51 APHA (bib1) 1998 Mariño, Bones, Kattla, Rudd (bib26) 2010; 6 Le, Kunacheva, Stuckey (bib22) 2016; 50 Dreywood (bib8) 1946; 18 Everette, Bryant, Green, Abbey, Wangila, Walker (bib10) 2010; 58 Wiechelman, Braun, Fitzpatrick (bib36) 1988; 175 Dische (bib6) 1950; 183 Li, Kisara, Danielsson, Lindström, Gellerstedt (bib24) 2007; 342 Filisetti-Cozzi, Carpita (bib12) 1991; 197 Rondel, Marcato-Romain, Girbal-Neuhauser (bib31) 2013; 47 Dubois, Gilles, Hamilton, Rebers, Smith (bib9) 1956; 28 Box (bib4) 1983; 17 Felz, Al-Zuhairy, Aarstad, van Loosdrecht, Lin (bib11) 2016 Le, Stuckey (bib23) 2016; 94 Balazs, Berntsen, Karossa, Swann (bib3) 1965; 12 Royal HaskoningDHV (bib32) 2018 Frølund, Griebe, Nielsen (bib15) 1995; 43 Pronk, Neu, van Loosdrecht, Lin (bib29) 2017; 122 Ras, Girbal-Neuhauser, Paul, Spérandio, Lefebvre (bib30) 2008; 42 Frølund, Palmgren, Keiding, Nielsen (bib16) 1996; 30 Kumirska, Czerwicka, Kaczyński, Bychowska, Brzozowski, Thöming, Stepnowski (bib21) 2010; 8 Myrold, Zeglin, Jansson (bib27) 2014; 78 Dell, Morris (bib5) 2001; 291 Kaltashov, Bobst, Abzalimov, Wang, Baykal, Wang (bib20) 2012; 30 Svensäter, Welin, Wilkins, Beighton, Hamilton (bib35) 2001; 205 Lowry, Rosebrough, Farr, Randal (bib25) 1951; 193 Prior, Wu, Schaich (bib28) 2005; 53 Dische (bib7) 1946; 167 Balazs (10.1016/j.watres.2019.03.068_bib3) 1965; 12 Sharma (10.1016/j.watres.2019.03.068_bib33) 1966; 14 Smith (10.1016/j.watres.2019.03.068_bib34) 1985; 150 Le (10.1016/j.watres.2019.03.068_bib22) 2016; 50 Li (10.1016/j.watres.2019.03.068_bib24) 2007; 342 Galambos (10.1016/j.watres.2019.03.068_bib17) 1967; 19 Felz (10.1016/j.watres.2019.03.068_bib11) 2016 Mariño (10.1016/j.watres.2019.03.068_bib26) 2010; 6 Dische (10.1016/j.watres.2019.03.068_bib6) 1950; 183 Rondel (10.1016/j.watres.2019.03.068_bib31) 2013; 47 Dubois (10.1016/j.watres.2019.03.068_bib9) 1956; 28 Folin (10.1016/j.watres.2019.03.068_bib14) 1927; 73 Wiechelman (10.1016/j.watres.2019.03.068_bib36) 1988; 175 Frølund (10.1016/j.watres.2019.03.068_bib15) 1995; 43 Lowry (10.1016/j.watres.2019.03.068_bib25) 1951; 193 Avella (10.1016/j.watres.2019.03.068_bib2) 2010; 408 Flemming (10.1016/j.watres.2019.03.068_bib13) 2010; 8 APHA (10.1016/j.watres.2019.03.068_bib1) 1998 Kaltashov (10.1016/j.watres.2019.03.068_bib20) 2012; 30 Le (10.1016/j.watres.2019.03.068_bib23) 2016; 94 Myrold (10.1016/j.watres.2019.03.068_bib27) 2014; 78 Box (10.1016/j.watres.2019.03.068_bib4) 1983; 17 Ikawa (10.1016/j.watres.2019.03.068_bib19) 2003; 51 Ras (10.1016/j.watres.2019.03.068_bib30) 2008; 42 Filisetti-Cozzi (10.1016/j.watres.2019.03.068_bib12) 1991; 197 Kumirska (10.1016/j.watres.2019.03.068_bib21) 2010; 8 Pronk (10.1016/j.watres.2019.03.068_bib29) 2017; 122 Prior (10.1016/j.watres.2019.03.068_bib28) 2005; 53 Frølund (10.1016/j.watres.2019.03.068_bib16) 1996; 30 Dreywood (10.1016/j.watres.2019.03.068_bib8) 1946; 18 Royal HaskoningDHV (10.1016/j.watres.2019.03.068_bib32) Dell (10.1016/j.watres.2019.03.068_bib5) 2001; 291 Everette (10.1016/j.watres.2019.03.068_bib10) 2010; 58 Garza (10.1016/j.watres.2019.03.068_bib18) 2015; 72 Svensäter (10.1016/j.watres.2019.03.068_bib35) 2001; 205 Dische (10.1016/j.watres.2019.03.068_bib7) 1946; 167 |
| References_xml | – start-page: 1 year: 2016 end-page: 8 ident: bib11 article-title: Extraction of structural extracellular polymeric substances from aerobic granular sludge publication-title: J. Vis. Exp. contributor: fullname: Lin – volume: 342 start-page: 1442 year: 2007 end-page: 1449 ident: bib24 article-title: An improved methodology for the quantification of uronic acid units in xylans and other polysaccharides publication-title: Carbohydr. Res. contributor: fullname: Gellerstedt – volume: 14 start-page: 11 year: 1966 end-page: 16 ident: bib33 article-title: Colorimetric estimation of humic acid with the phenol reagent of Folin and Ciocalteu publication-title: Anal. Biochem. contributor: fullname: Krishnan – volume: 6 start-page: 713 year: 2010 end-page: 723 ident: bib26 article-title: A systematic approach to protein glycosylation analysis: a path through the maze publication-title: Nat. Chem. Biol. contributor: fullname: Rudd – volume: 291 start-page: 2351 year: 2001 end-page: 2356 ident: bib5 article-title: Glycoprotein structure determination by mass spectrometry publication-title: Science (80-. ) contributor: fullname: Morris – volume: 42 start-page: 1867 year: 2008 end-page: 1878 ident: bib30 article-title: Protein extraction from activated sludge: an analytical approach publication-title: Water Res. contributor: fullname: Lefebvre – volume: 78 start-page: 3 year: 2014 end-page: 10 ident: bib27 article-title: The potential of metagenomic approaches for understanding soil microbial processes publication-title: Soil Sci. Soc. Am. J. contributor: fullname: Jansson – year: 2018 ident: bib32 article-title: Nereda WWTP in Dinxperlo, The Netherlands contributor: fullname: Royal HaskoningDHV – volume: 175 start-page: 231 year: 1988 end-page: 237 ident: bib36 article-title: Investigation of the Bicinchonicin Acid protein assay: identification of the groups responsible for color formation publication-title: Anal. Biochem. contributor: fullname: Fitzpatrick – volume: 12 start-page: 547 year: 1965 end-page: 558 ident: bib3 article-title: An automated method for the determination of hexuronic acids publication-title: Anal. Chem. contributor: fullname: Swann – volume: 205 start-page: 139 year: 2001 end-page: 146 ident: bib35 article-title: Protein expression by planktonic and biofilm cells of Streptococcus mutans publication-title: FEMS Microbiol. Lett. contributor: fullname: Hamilton – volume: 30 start-page: 1749 year: 1996 end-page: 1758 ident: bib16 article-title: Extraction of extracellular polymers from activated sludge using a cation exchange resin publication-title: Water Res. contributor: fullname: Nielsen – volume: 94 start-page: 280 year: 2016 end-page: 287 ident: bib23 article-title: Colorimetric measurement of carbohydrates in biological wastewater treatment systems: a critical evaluation publication-title: Water Res. contributor: fullname: Stuckey – volume: 43 start-page: 755 year: 1995 end-page: 761 ident: bib15 article-title: Enzymatic activity in the activated sludge flocmatrix publication-title: Appl. Microbiol. Biotechnol. contributor: fullname: Nielsen – volume: 183 start-page: 489 year: 1950 end-page: 495 ident: bib6 article-title: A modification of the carbazole reaction of hexuronic acids for the study of polyuronides publication-title: J. Biol. Chem. contributor: fullname: Dische – volume: 150 start-page: 76 year: 1985 end-page: 85 ident: bib34 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. contributor: fullname: Klenk – volume: 17 start-page: 511 year: 1983 end-page: 525 ident: bib4 article-title: Investigation of the Folin-Ciocalteu phenol reagent for the determiantion of polyphenolic substances in natural waters publication-title: Water Res. contributor: fullname: Box – volume: 58 start-page: 8139 year: 2010 end-page: 8144 ident: bib10 article-title: Thorough study of reactivity of various compound classes toward the folin-Ciocalteu reagent publication-title: J. Agric. Food Chem. contributor: fullname: Walker – volume: 51 start-page: 1811 year: 2003 end-page: 1815 ident: bib19 article-title: Utilization of folin-ciocalteu phenol reagent for the detection of certain nitrogen compounds publication-title: J. Agric. Food Chem. contributor: fullname: Sasner – volume: 167 start-page: 189 year: 1946 end-page: 198 ident: bib7 article-title: A new specific color reaction of hexuronic acids publication-title: J. Biol. Chem. contributor: fullname: Dische – volume: 8 start-page: 623 year: 2010 end-page: 633 ident: bib13 article-title: The biofilm matrix publication-title: Nat. Rev. Microbiol. contributor: fullname: Wingender – volume: 408 start-page: 4906 year: 2010 end-page: 4909 ident: bib2 article-title: The pitfalls of protein quantification in wastewater treatment studies publication-title: Sci. Total Environ. contributor: fullname: de Donato – volume: 28 start-page: 350 year: 1956 end-page: 356 ident: bib9 article-title: Colorimetric method for determination of sugars and related substances publication-title: Anal. Chem. contributor: fullname: Smith – volume: 18 start-page: 499 year: 1946 ident: bib8 article-title: Qualitative test for carbohydrate publication-title: Material. Ind. Eng. Chem. - Anal. Ed. contributor: fullname: Dreywood – volume: 193 start-page: 265 year: 1951 end-page: 275 ident: bib25 article-title: Protein measurement with the folin phenol reagent publication-title: J. Biol. Chem. contributor: fullname: Randal – volume: 122 start-page: 148 year: 2017 end-page: 158 ident: bib29 article-title: The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp publication-title: Water Res. contributor: fullname: Lin – year: 1998 ident: bib1 article-title: Standard Methods for the Examination of Water and Wastewater contributor: fullname: APHA – volume: 72 start-page: 4287 year: 2015 end-page: 4308 ident: bib18 article-title: From cultured to uncultured genome sequences: metagenomics and modeling microbial ecosystems publication-title: Cell. Mol. Life Sci. contributor: fullname: Dutilh – volume: 8 start-page: 1567 year: 2010 end-page: 1636 ident: bib21 article-title: Application of spectroscopic methods for structural analysis of chitin and chitosan publication-title: Mar. Drugs contributor: fullname: Stepnowski – volume: 197 start-page: 157 year: 1991 end-page: 162 ident: bib12 article-title: Measurement of uronic acids without interference from neutral sugars publication-title: Anal. Biochem. contributor: fullname: Carpita – volume: 53 start-page: 4290 year: 2005 end-page: 4302 ident: bib28 article-title: Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements publication-title: J. Agric. Food Chem. contributor: fullname: Schaich – volume: 19 start-page: 133 year: 1967 end-page: 143 ident: bib17 article-title: The reaction of carbazole with carbohydrates publication-title: Anal. Biochem. contributor: fullname: Galambos – volume: 50 start-page: 3074 year: 2016 end-page: 3081 ident: bib22 article-title: “Protein” measurement in biological wastewater treatment systems: a critical evaluation publication-title: Environ. Sci. Technol. contributor: fullname: Stuckey – volume: 47 start-page: 2901 year: 2013 end-page: 2908 ident: bib31 article-title: Development and validation of a colorimetric assay for simultaneous quantification of neutral and uronic sugars publication-title: Water Res. contributor: fullname: Girbal-Neuhauser – volume: 30 start-page: 210 year: 2012 end-page: 222 ident: bib20 article-title: Advances and challenges in analytical characterization of biotechnology products: mass spectrometry-based approaches to study properties and behavior of protein therapeutics publication-title: Biotechnol. Adv. contributor: fullname: Wang – volume: 73 start-page: 627 year: 1927 end-page: 648 ident: bib14 article-title: On tyrosine and tryptophane in proteins publication-title: J. Biol. Chem. contributor: fullname: Ciocalteau – volume: 53 start-page: 4290 year: 2005 ident: 10.1016/j.watres.2019.03.068_bib28 article-title: Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements publication-title: J. Agric. Food Chem. doi: 10.1021/jf0502698 contributor: fullname: Prior – volume: 42 start-page: 1867 year: 2008 ident: 10.1016/j.watres.2019.03.068_bib30 article-title: Protein extraction from activated sludge: an analytical approach publication-title: Water Res. doi: 10.1016/j.watres.2007.11.011 contributor: fullname: Ras – volume: 73 start-page: 627 year: 1927 ident: 10.1016/j.watres.2019.03.068_bib14 article-title: On tyrosine and tryptophane in proteins publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)84277-6 contributor: fullname: Folin – volume: 183 start-page: 489 year: 1950 ident: 10.1016/j.watres.2019.03.068_bib6 article-title: A modification of the carbazole reaction of hexuronic acids for the study of polyuronides publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)51173-5 contributor: fullname: Dische – volume: 342 start-page: 1442 year: 2007 ident: 10.1016/j.watres.2019.03.068_bib24 article-title: An improved methodology for the quantification of uronic acid units in xylans and other polysaccharides publication-title: Carbohydr. Res. doi: 10.1016/j.carres.2007.03.031 contributor: fullname: Li – volume: 197 start-page: 157 year: 1991 ident: 10.1016/j.watres.2019.03.068_bib12 article-title: Measurement of uronic acids without interference from neutral sugars publication-title: Anal. Biochem. doi: 10.1016/0003-2697(91)90372-Z contributor: fullname: Filisetti-Cozzi – volume: 291 start-page: 2351 year: 2001 ident: 10.1016/j.watres.2019.03.068_bib5 article-title: Glycoprotein structure determination by mass spectrometry publication-title: Science (80-. ) doi: 10.1126/science.1058890 contributor: fullname: Dell – volume: 12 start-page: 547 year: 1965 ident: 10.1016/j.watres.2019.03.068_bib3 article-title: An automated method for the determination of hexuronic acids publication-title: Anal. Chem. contributor: fullname: Balazs – volume: 408 start-page: 4906 year: 2010 ident: 10.1016/j.watres.2019.03.068_bib2 article-title: The pitfalls of protein quantification in wastewater treatment studies publication-title: Sci. Total Environ. doi: 10.1016/j.scitotenv.2010.05.039 contributor: fullname: Avella – volume: 72 start-page: 4287 year: 2015 ident: 10.1016/j.watres.2019.03.068_bib18 article-title: From cultured to uncultured genome sequences: metagenomics and modeling microbial ecosystems publication-title: Cell. Mol. Life Sci. doi: 10.1007/s00018-015-2004-1 contributor: fullname: Garza – ident: 10.1016/j.watres.2019.03.068_bib32 contributor: fullname: Royal HaskoningDHV – volume: 19 start-page: 133 year: 1967 ident: 10.1016/j.watres.2019.03.068_bib17 article-title: The reaction of carbazole with carbohydrates publication-title: Anal. Biochem. doi: 10.1016/0003-2697(67)90142-X contributor: fullname: Galambos – volume: 175 start-page: 231 year: 1988 ident: 10.1016/j.watres.2019.03.068_bib36 article-title: Investigation of the Bicinchonicin Acid protein assay: identification of the groups responsible for color formation publication-title: Anal. Biochem. doi: 10.1016/0003-2697(88)90383-1 contributor: fullname: Wiechelman – volume: 8 start-page: 1567 year: 2010 ident: 10.1016/j.watres.2019.03.068_bib21 article-title: Application of spectroscopic methods for structural analysis of chitin and chitosan publication-title: Mar. Drugs doi: 10.3390/md8051567 contributor: fullname: Kumirska – volume: 122 start-page: 148 year: 2017 ident: 10.1016/j.watres.2019.03.068_bib29 article-title: The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp publication-title: Water Res. doi: 10.1016/j.watres.2017.05.068 contributor: fullname: Pronk – volume: 58 start-page: 8139 year: 2010 ident: 10.1016/j.watres.2019.03.068_bib10 article-title: Thorough study of reactivity of various compound classes toward the folin-Ciocalteu reagent publication-title: J. Agric. Food Chem. doi: 10.1021/jf1005935 contributor: fullname: Everette – volume: 50 start-page: 3074 year: 2016 ident: 10.1016/j.watres.2019.03.068_bib22 article-title: “Protein” measurement in biological wastewater treatment systems: a critical evaluation publication-title: Environ. Sci. Technol. doi: 10.1021/acs.est.5b05261 contributor: fullname: Le – volume: 8 start-page: 623 year: 2010 ident: 10.1016/j.watres.2019.03.068_bib13 article-title: The biofilm matrix publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro2415 contributor: fullname: Flemming – volume: 6 start-page: 713 year: 2010 ident: 10.1016/j.watres.2019.03.068_bib26 article-title: A systematic approach to protein glycosylation analysis: a path through the maze publication-title: Nat. Chem. Biol. doi: 10.1038/nchembio.437 contributor: fullname: Mariño – volume: 150 start-page: 76 year: 1985 ident: 10.1016/j.watres.2019.03.068_bib34 article-title: Measurement of protein using bicinchoninic acid publication-title: Anal. Biochem. doi: 10.1016/0003-2697(85)90442-7 contributor: fullname: Smith – year: 1998 ident: 10.1016/j.watres.2019.03.068_bib1 contributor: fullname: APHA – volume: 18 start-page: 499 year: 1946 ident: 10.1016/j.watres.2019.03.068_bib8 article-title: Qualitative test for carbohydrate publication-title: Material. Ind. Eng. Chem. - Anal. Ed. doi: 10.1021/i560156a015 contributor: fullname: Dreywood – volume: 94 start-page: 280 year: 2016 ident: 10.1016/j.watres.2019.03.068_bib23 article-title: Colorimetric measurement of carbohydrates in biological wastewater treatment systems: a critical evaluation publication-title: Water Res. doi: 10.1016/j.watres.2016.03.008 contributor: fullname: Le – volume: 167 start-page: 189 year: 1946 ident: 10.1016/j.watres.2019.03.068_bib7 article-title: A new specific color reaction of hexuronic acids publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)35155-4 contributor: fullname: Dische – volume: 78 start-page: 3 year: 2014 ident: 10.1016/j.watres.2019.03.068_bib27 article-title: The potential of metagenomic approaches for understanding soil microbial processes publication-title: Soil Sci. Soc. Am. J. doi: 10.2136/sssaj2013.07.0287dgs contributor: fullname: Myrold – volume: 17 start-page: 511 year: 1983 ident: 10.1016/j.watres.2019.03.068_bib4 article-title: Investigation of the Folin-Ciocalteu phenol reagent for the determiantion of polyphenolic substances in natural waters publication-title: Water Res. doi: 10.1016/0043-1354(83)90111-2 contributor: fullname: Box – volume: 30 start-page: 1749 year: 1996 ident: 10.1016/j.watres.2019.03.068_bib16 article-title: Extraction of extracellular polymers from activated sludge using a cation exchange resin publication-title: Water Res. doi: 10.1016/0043-1354(95)00323-1 contributor: fullname: Frølund – volume: 205 start-page: 139 year: 2001 ident: 10.1016/j.watres.2019.03.068_bib35 article-title: Protein expression by planktonic and biofilm cells of Streptococcus mutans publication-title: FEMS Microbiol. Lett. doi: 10.1111/j.1574-6968.2001.tb10937.x contributor: fullname: Svensäter – volume: 193 start-page: 265 year: 1951 ident: 10.1016/j.watres.2019.03.068_bib25 article-title: Protein measurement with the folin phenol reagent publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)52451-6 contributor: fullname: Lowry – volume: 30 start-page: 210 year: 2012 ident: 10.1016/j.watres.2019.03.068_bib20 article-title: Advances and challenges in analytical characterization of biotechnology products: mass spectrometry-based approaches to study properties and behavior of protein therapeutics publication-title: Biotechnol. Adv. doi: 10.1016/j.biotechadv.2011.05.006 contributor: fullname: Kaltashov – volume: 51 start-page: 1811 year: 2003 ident: 10.1016/j.watres.2019.03.068_bib19 article-title: Utilization of folin-ciocalteu phenol reagent for the detection of certain nitrogen compounds publication-title: J. Agric. Food Chem. doi: 10.1021/jf021099r contributor: fullname: Ikawa – volume: 14 start-page: 11 year: 1966 ident: 10.1016/j.watres.2019.03.068_bib33 article-title: Colorimetric estimation of humic acid with the phenol reagent of Folin and Ciocalteu publication-title: Anal. Biochem. doi: 10.1016/0003-2697(66)90050-9 contributor: fullname: Sharma – volume: 28 start-page: 350 year: 1956 ident: 10.1016/j.watres.2019.03.068_bib9 article-title: Colorimetric method for determination of sugars and related substances publication-title: Anal. Chem. doi: 10.1021/ac60111a017 contributor: fullname: Dubois – start-page: 1 year: 2016 ident: 10.1016/j.watres.2019.03.068_bib11 article-title: Extraction of structural extracellular polymeric substances from aerobic granular sludge publication-title: J. Vis. Exp. contributor: fullname: Felz – volume: 43 start-page: 755 year: 1995 ident: 10.1016/j.watres.2019.03.068_bib15 article-title: Enzymatic activity in the activated sludge flocmatrix publication-title: Appl. Microbiol. Biotechnol. doi: 10.1007/s002530050481 contributor: fullname: Frølund – volume: 47 start-page: 2901 year: 2013 ident: 10.1016/j.watres.2019.03.068_bib31 article-title: Development and validation of a colorimetric assay for simultaneous quantification of neutral and uronic sugars publication-title: Water Res. doi: 10.1016/j.watres.2013.03.010 contributor: fullname: Rondel |
| SSID | ssj0002239 |
| Score | 2.6940079 |
| Snippet | Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits... |
| SourceID | proquest crossref pubmed elsevier |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 201 |
| SubjectTerms | Aerobic granular sludge Biofilm Colorimetric methods EPS analysis Extracellular polymeric substances (EPS) |
| Title | Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS) |
| URI | https://dx.doi.org/10.1016/j.watres.2019.03.068 https://www.ncbi.nlm.nih.gov/pubmed/30953855 https://search.proquest.com/docview/2204692703 |
| Volume | 157 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LSwMxEA6lXvQgvq2PEsGDHtZuN8k-jqVUqmIRtNBb2DwWKmW30Ip48bc7s9mtCorgcZdkN0wmM1_IN18IOe8qnvKE-V4CydHjItNeYpLQM0nKMxEoG2msRr4fhcMxv52ISYP061oYpFVWsd_F9DJaV286lTU78-kUa3wh-THBAYIA5kkmWMEO6Q98-ur9k-YB6S-pT5mxdV0-V3K8XlMsyECCl5M6RcHVn9PTb_CzTEPXW2Szwo-054a4TRo23yEbX1QFd8m0FgGgeqXG7IotqbsvekEBqVJAfjStJElokVGnJIsqHBQCNvSzsxlyVOm8mL2V5zp0AVFmiW6yoBeDh8fLPTK-Hjz1h151o4KnecSXXsQseE4cqtSksNFhgVWRhnVoGGeKa9iOqEhp1PiKYm2UMgJv7EtMplRmTKzZPmnmRW4PCUXoB9_UATec2zSLu74OuQ1EHGhfB2GLeLUh5dwJZ8iaUfYsneElGl76TILhWySqrS2_OYCE2P5Hz7N6ciSsDbROmtviBRoFuPsPIKi1yIGbtdVYGArtxUIc_fu_x2Qdn5A31hUnpAmzZE8BoSxVu3TBNlnr3dwNRx-Xrefm |
| link.rule.ids | 315,783,787,4509,24128,27936,27937,45597,45691 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LSwMxEB58HNSD-LY-I3jQw2q7SfZxlKLUVxFsobeweSxUSltoRbz4253Z7PoARfC6m-yGyWTmC_nmC8BxQ4tMpLwepJgcAyFzE6Q2jQKbZiKXoXaxoWrk-3bU6oqbnuzNQLOqhSFaZRn7fUwvonX55Ly05vm436caX0x-XAqEIIh50t4szAvCx-jUZ2-fPA_Mf2l1zEzNq_q5guT1klFFBjG8vNYpKa7-nJ9-w59FHrpageUSQLILP8ZVmHHDNVj6Iiu4Dv1KBYCZDzlmX23J_IXRE4ZQlSH0Y1mpScJGOfNSsiTDwTBiYz83GBBJlY1Hg9fiYIdNMMxMyU8m7OTy4fF0A7pXl51mKyivVAiMiMU0iLlD10kindkMdzo8dDo2uBAtF1wLg_sRHWtDIl9xYqzWVtKVfanNtc6tTQzfhLnhaOi2gRH2w2-aUFghXJYnjbqJhAtlEpq6CaMaBJUh1dgrZ6iKUvakvOEVGV7VuULD1yCurK2-eYDC4P5Hz6NqchQuDrJONnSjZ2wU0vY_xKhWgy0_ax9j4aS0l0i58-__HsJCq3N_p-6u27e7sEhviETWkHswhzPm9hGuTPVB4Y7vDnzpfw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Chemical+characterization+methods+for+the+analysis+of+structural+extracellular+polymeric+substances+%28EPS%29&rft.jtitle=Water+research+%28Oxford%29&rft.au=Felz%2C+Simon&rft.au=Vermeulen%2C+Pascalle&rft.au=van+Loosdrecht%2C+Mark+C.M.&rft.au=Lin%2C+Yue+Mei&rft.date=2019-06-15&rft.issn=0043-1354&rft.volume=157&rft.spage=201&rft.epage=208&rft_id=info:doi/10.1016%2Fj.watres.2019.03.068&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_watres_2019_03_068 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0043-1354&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0043-1354&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0043-1354&client=summon |