Diversity of rhizobia, symbiotic effectiveness, and potential of inoculation in Acacia mearnsii seedling production

• Published in: Brazilian journal of microbiology Vol. 54; no. 1; pp. 335 - 348
• Main Authors: de São José, Jackson Freitas Brilhante, Hernandes, Marcos André Santos, Volpiano, Camila Gazolla, Lisboa, Bruno Brito, Beneduzi, Anelise, Bayer, Cimelio, Simon, Augusto Arlindo, de Oliveira, Jeferson, Passaglia, Luciane Maria Pereira, Vargas, Luciano Kayser
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2023; Springer Nature B.V
• Subjects: Acacia; Acacia mearnsii; Biomedical and Life Sciences; Bradyrhizobium; Bradyrhizobium - genetics; Economic importance; Efficiency; Food Microbiology; Genetic diversity; Germination; Inoculation; Legumes; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Nitrogen; Nitrogen Fixation; Nodulation; Nodules; Phylogeny; Plantations; Principal components analysis; Rhizobium - genetics; Root Nodules, Plant - microbiology; Seedlings; Soil and Agricultural Microbiology - Research Paper; Symbiosis; Symbiosis - genetics; Wattle; Brazil; Black wattle; Rhizobium; Bacterial diversity; Biological nitrogen fixation
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-022-00867-2

Black wattle ( Acacia mearnsii ) is a forest species of significant economic importance in southern Brazil; as a legume, it forms symbiotic associations with rhizobia, fixing atmospheric nitrogen. Nonetheless, little is known about native rhizobia in soils where the species is cultivated. Therefore, this study aimed to evaluate the diversity and symbiotic efficiency of rhizobia nodulating A. mearnsii in commercial planting areas and validate the efficiency of a potential strain in promoting seedling development. To this end, nodules were collected from four A. mearnsii commercial plantations located in Rio Grande do Sul State, southern Brazil. A total of 80 rhizobia isolates were obtained from black wattle nodules, and thirteen clusters were obtained by rep-PCR. Higher genetic diversity was found within the rhizobial populations from the Duas Figueiras (H′ = 2.224) and Seival (H′ = 2.112) plantations. Twelve isolates were evaluated belonging to the genus Bradyrhizobium, especially to the species Bradyrhizobium guangdongense. The principal component analysis indicated an association between rhizobia diversity and the content of clay, Ca, Mg, and K. Isolates and reference strains (SEMIA 6163 and 6164) induced nodulation and fixed N via symbiosis with black wattle plants after 60 days of germination. The isolates DF2.4, DF2.3, DF3.3, SEMIA 6164, SEMIA 6163, CA4.3, OV3.4, and OV1.4 showed shoot nitrogen accumulation values similar to the N + control treatment. In the second experiment (under nursery conditions), inoculation with the reference strain SEMIA 6164 generally improved the growth of A. mearnsii seedlings, reinforcing its efficiency even under production conditions.