Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination

• Published in: Nucleic acids research Vol. 42; no. 4; p. e23
• Main Authors: Colloms, Sean D, Merrick, Christine A, Olorunniji, Femi J, Stark, W Marshall, Smith, Margaret C M, Osbourn, Anne, Keasling, Jay D, Rosser, Susan J
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2014
• Subjects: Bacteriophages - enzymology; BASIC BIOLOGICAL SCIENCES; Biochemistry & Molecular Biology; Biosynthetic Pathways - genetics; Cloning, Molecular - methods; Gene Order; Integrases - metabolism; Metabolic Engineering - methods; Metabolic Networks and Pathways - genetics; Methods Online; Recombination, Genetic; Ribosomes - metabolism; Synthetic Biology - methods; Synthetic Biology and Assembly Cloning

Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by C31 integrase. Using six orthogonal attP/attB recombination site pair...