A Novel Dual Antibody Staining Assay to Measure Estrogen Receptor Transcriptional Activity

• Published in: Journal of fluorescence Vol. 31; no. 1; pp. 219 - 227
• Main Authors: van Hemert, Freek, Dam-de Veen, Christa, Konings, Sil, van der Ven, John, van de Stolpe, Anja
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.01.2021; Springer Nature B.V
• Subjects: Algorithms; Analytical Chemistry; Antibodies; Antibodies, Monoclonal - immunology; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Nucleus - metabolism; Digital imaging; Estrogens; Fluorescence; Humans; MCF-7 Cells; Monoclonal antibodies; Nuclei (cytology); Original; Original Article; Receptors; Receptors, Estrogen - metabolism; Staining; Staining and Labeling; Transcription, Genetic; Estrogen receptor; Transcription factor complex; Immunocytochemistry; ER pathway; Pathway signalling

Activity of the canonical estrogen receptor (ER) pathway is equivalent to functional activity of the nuclear ER transcription factor. Monoclonal antibodies (MoAbs) that identify nuclear ER in cells and tissue samples are frequently used to assess ER transcriptional activity, however, it remains uncl...