Regulation of Rep helicase unwinding by an auto-inhibitory subdomain

• Published in: Nucleic acids research Vol. 47; no. 5; pp. 2523 - 2532
• Main Authors: Makurath, Monika A, Whitley, Kevin D, Nguyen, Binh, Lohman, Timothy M, Chemla, Yann R
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 18.03.2019
• Subjects: Adenosine Triphosphatases - genetics; DNA - chemistry; DNA - genetics; DNA Helicases - chemistry; DNA Helicases - genetics; DNA Replication - genetics; DNA, Single-Stranded; Escherichia coli - enzymology; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Kinetics; Models, Molecular; Mutation - genetics; Nucleic Acid Conformation; Nucleic Acid Enzymes; Protein Domains - genetics

Abstract Helicases are biomolecular motors that unwind nucleic acids, and their regulation is essential for proper maintenance of genomic integrity. Escherichia coli Rep helicase, whose primary role is to help restart stalled replication, serves as a model for Superfamily I helicases. The activity o...