Targeted epigenetic editing of SPDEF reduces mucus production in lung epithelial cells

• Published in: American journal of physiology. Lung cellular and molecular physiology Vol. 312; no. 3; pp. L334 - L347
• Main Authors: Song, Juan, Cano-Rodriquez, David, Winkle, Melanie, Gjaltema, Rutger A F, Goubert, Désirée, Jurkowski, Tomasz P, Heijink, Irene H, Rots, Marianne G, Hylkema, Machteld N
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.03.2017
• Subjects: Base Sequence; Body fluids; Cell Line; DNA (Cytosine-5-)-Methyltransferases - genetics; DNA (Cytosine-5-)-Methyltransferases - metabolism; DNA Methylation - genetics; Down-Regulation - genetics; Epigenesis, Genetic; Epigenetics; Epithelial Cells - metabolism; Gene Editing; Gene expression; Gene Silencing; Histocompatibility Antigens - genetics; Histocompatibility Antigens - metabolism; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Humans; Lung - cytology; Lung diseases; Models, Biological; Mucin 5AC - metabolism; Mucus - metabolism; Physiology; Promoter Regions, Genetic - genetics; Protein Domains; Proto-Oncogene Proteins c-ets - genetics; Proto-Oncogene Proteins c-ets - metabolism; Quality of life; Zinc Fingers; epigenetic editing; DNA methylation; mucus production; SPDEF

Airway mucus hypersecretion contributes to the morbidity and mortality in patients with chronic inflammatory lung diseases. Reducing mucus production is crucial for improving patients' quality of life. The transcription factor SAM-pointed domain-containing Ets-like factor ( ) plays a critical r...