CTCF functions as an insulator for somatic genes and a chromatin remodeler for pluripotency genes during reprogramming

• Published in: Cell reports (Cambridge) Vol. 39; no. 1; p. 110626
• Main Authors: Song, Yawei, Liang, Zhengyu, Zhang, Jie, Hu, Gongcheng, Wang, Juehan, Li, Yaoyi, Guo, Rong, Dong, Xiaotao, Babarinde, Isaac A., Ping, Wangfang, Sheng, Ying-Liang, Li, Huanhuan, Chen, Zhaoming, Gao, Minghui, Chen, Yang, Shan, Ge, Zhang, Michael Q., Hutchins, Andrew P., Fu, Xiang-Dong, Yao, Hongjie
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.04.2022; Elsevier
• Subjects: Animals; CCCTC-Binding Factor - genetics; CCCTC-Binding Factor - metabolism; Cellular Reprogramming - genetics; Chromatin; chromatin accessibility; chromatin insulation; CP: Molecular biology; CP: Stem cell research; CTCF; Enhancer Elements, Genetic - genetics; enhancer-promoter interactions; Humans; Mice; Promoter Regions, Genetic; reprogramming; SMARCA5; reprogramming; CP: Stem cell research; chromatin accessibility; SMARCA5; CP: Molecular biology; CTCF; chromatin insulation; enhancer-promoter interactions

CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression is progressively increased during induced pluripo...