Effects of epidermal growth factor and hormones on granulosa expansion and nuclear maturation of dog oocytes in vitro

• Published in: Theriogenology Vol. 65; no. 6; pp. 1037 - 1047
• Main Authors: Bolamba, Digbo, Russ, Kara D., Harper, Sally A., Sandler, Jody L., Durrant, Barbara S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2006
• Subjects: Animals; Cell Nucleus - physiology; Cells, Cultured; culture media; cumulus oophorus; Dogs; EGF; epidermal growth factor; Epidermal Growth Factor - pharmacology; estradiol; Female; follicle-stimulating hormone; FSH; Granulosa; granulosa cells; Granulosa Cells - drug effects; Granulosa Cells - physiology; Hormones - pharmacology; luteinizing hormone; oocytes; Oocytes - drug effects; Oocytes - ultrastructure; Ovarian Follicle - cytology; ovarian follicles; FSH; Granulosa; LH; EGF; E2

Gonadotropins, steroids and growth factors stimulate or inhibit cumulus expansion, nuclear maturation, or both, of most mammalian oocytes in vitro. The objective was to evaluate the effects of epidermal growth factor (EGF) and various hormone combinations on in vitro granulosa/cumulus (G-C) expansio...