Retinal artery occlusion is associated with compositional and functional shifts in the gut microbiome and altered trimethylamine-N-oxide levels

• Published in: Scientific reports Vol. 9; no. 1; pp. 15303 - 11
• Main Authors: Zysset-Burri, Denise C., Keller, Irene, Berger, Lieselotte E., Neyer, Peter J., Steuer, Christian, Wolf, Sebastian, Zinkernagel, Martin S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.10.2019; Nature Publishing Group
• Subjects: 38; 38/23; 38/39; 38/43; 692/499; 692/699/3161/3175; Abundance; Actinobacteria - isolation & purification; Aged; Arteriosclerosis; Atherosclerosis; Atherosclerosis - metabolism; Bacteroides - isolation & purification; Bifidobacterium - isolation & purification; Cardiovascular disease; Cardiovascular diseases; Cholesterol; Digestive system; Faecalibacterium - isolation & purification; Female; Fluorescein Angiography - methods; Gastrointestinal Microbiome; Gut microbiota; Humanities and Social Sciences; Humans; Intestinal microflora; Lachnospiraceae; Lipid metabolism; Male; Metabolites; Methylamines - metabolism; Microbiomes; Microbiota; multidisciplinary; Occlusion; Relative abundance; Retina; Retinal Artery Occlusion - diagnosis; Retinal Artery Occlusion - metabolism; Retinal Artery Occlusion - microbiology; Risk Factors; Science; Science (multidisciplinary); Tomography, Optical Coherence - methods; Trimethylamine; Trimethylamine-N-oxide
• DOI: 10.1038/s41598-019-51698-5
• ISSN: 2045-2322

Retinal artery occlusion (RAO) is a sight threatening complication of cardiovascular disease and commonly occurs due to underlying atherosclerosis. As cardiovascular disease and atherosclerosis in particular has been associated with compositional alterations in the gut microbiome, we investigated this association in patients with clinically confirmed non-arteritic RAO compared to age- and sex-matched controls. On the phylum level, the relative abundance of Bacteroidetes was decreased in patients with RAO compared to controls, whereas the opposite applied for the phylum of Proteobacteria . Several genera and species such as Actinobacter , Bifidobacterium spp ., Bacteroides stercoris , Faecalibacterium prausnitzii were relatively enriched in patients with RAO, whereas others such as Odoribacter , Parasutterella or Lachnospiraceae were significantly lower. Patient’s gut microbiomes were enriched in genes of the cholesterol metabolism pathway. The gut derived, pro-atherogenic metabolite trimethylamine-N-oxide (TMAO) was significantly higher in patients with RAO compared to controls (p = 0.023) and a negative correlation between relative abundances of genera Parasutterella and Lachnospiraceae and TMAO levels and a positive correlation between relative abundance of genus Akkermansia and TMAO levels was found in study subjects. Our findings proposes that RAO is associated with alterations in the gut microbiome and with elevated TMAO levels, suggesting that RAO could be targeted by microbiome-altering interventions.