A Lateral Flow Assay for Nucleic Acid Detection Based on Rolling Circle Amplification Using Capture Ligand-Modified Oligonucleotides

• Published in: Biochip journal Vol. 16; no. 4; pp. 441 - 450
• Main Authors: Lee, Ha Neul, Lee, Juhee, Kang, Yoo Kyung, Lee, Joo Hoon, Yang, Seungju, Chung, Hyun Jung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean BioChip Society (KBCS) 01.12.2022; Springer Nature B.V
• Subjects: Acids; Amplification; Antibiotic resistance; Antibiotics; Antibodies; Assaying; Binding; Biomedical Engineering and Bioengineering; Biotechnology; Chemistry; Chemistry and Materials Science; Colorimetry; Deoxyribonucleic acid; Design; DNA; DNA polymerase; DNA probes; DNA-directed RNA polymerase; Drug resistance; Genetic screening; Genetic testing; Hybridization; Ligands; MecA protein; Methicillin; Nanoparticles; Nosocomial infections; Nucleic acids; Oligonucleotides; Original; Original Article; Point of care testing; Ribonucleic acid; RNA; RNA probes; RNA-directed RNA polymerase; Severe acute respiratory syndrome coronavirus 2; Staphylococcus infections; Substrates; Target detection; Thermal cycling; South Korea; Rapid diagnosis; Lateral flow assay; Genetic testing; Rolling circle amplification; Point-of-care

We introduce a lateral flow assay (LFA) integrated with a modified isothermal nucleic acid amplification procedure for rapid and simple genetic testing. Padlock probes specific for the target DNA were designed for ligation, followed by rolling circle amplification (RCA) using capture ligand-modified...