Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams

• Published in: International journal of radiation oncology, biology, physics Vol. 74; no. 4; pp. 1226 - 1235
• Main Authors: Ibañez, Irene L., M.D., M.Sc, Bracalente, Candelaria, M.Sc, Molinari, Beatriz L., Ph.D, Palmieri, Mónica A., M.Sc, Policastro, Lucía, Ph.D, Kreiner, Andrés J., Ph.D, Burlón, Alejandro A., Ph.D, Valda, Alejandro, Ph.D, Navalesi, Daniela, Davidson, Jorge, Ph.D, Davidson, Miguel, Ph.D, Vázquez, Mónica, B.S, Ozafrán, Mabel, Ph.D, Durán, Hebe, Ph.D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.07.2009
• Subjects: BEAMS; Biomarkers - analysis; Biomarkers - metabolism; Cell Survival; DNA; DNA Breaks, Double-Stranded; DNA DSB; DNA Repair; Dose-Response Relationship, Radiation; GAMMA SOURCES; Hematology, Oncology and Palliative Medicine; Histones - analysis; Histones - metabolism; Humans; Ionizing radiation; IONIZING RADIATIONS; IRRADIATION; LET; Linear Energy Transfer; LITHIUM; Lithium - therapeutic use; Melanoma cells; Melanoma, Experimental - genetics; Melanoma, Experimental - metabolism; Melanoma, Experimental - radiotherapy; MELANOMAS; PHOSPHORYLATION; Proton Therapy; PROTONS; RADIATION DOSES; Radiation Tolerance; Radioisotopes - therapeutic use; Radiology; RADIOLOGY AND NUCLEAR MEDICINE; RADIOSENSITIVITY; STRAND BREAKS; SURVIVAL CURVES; γH2AX; Ionizing radiation; LET; Melanoma cells; γH2AX; DNA DSB

Purpose The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irra...