Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus

• Published in: Molecular cell Vol. 56; no. 4; pp. 506 - 517
• Main Authors: Tamulaitis, Gintautas, Kazlauskiene, Migle, Manakova, Elena, Venclovas, Česlovas, Nwokeoji, Alison O., Dickman, Mark J., Horvath, Philippe, Siksnys, Virginijus
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.11.2014
• Subjects: Amino Acid Sequence; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Associated Proteins - chemistry; CRISPR-Associated Proteins - genetics; CRISPR-Associated Proteins - metabolism; Endoribonucleases - genetics; Endoribonucleases - metabolism; Molecular Sequence Data; Protein Binding; Protein Structure, Quaternary; RNA Cleavage; Scattering, Small Angle; Streptococcus thermophilus - enzymology; Streptococcus thermophilus - genetics; X-Ray Diffraction

Immunity against viruses and plasmids provided by CRISPR-Cas systems relies on a ribonucleoprotein effector complex that triggers the degradation of invasive nucleic acids (NA). Effector complexes of type I (Cascade) and II (Cas9-dual RNA) target foreign DNA. Intriguingly, the genetic evidence sugge...