Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging

• Published in: Nature methods Vol. 19; no. 5; pp. 554 - 559
• Main Authors: Chung, Kenny K. H., Zhang, Zhao, Kidd, Phylicia, Zhang, Yongdeng, Williams, Nathan D., Rollins, Bennett, Yang, Yang, Lin, Chenxiang, Baddeley, David, Bewersdorf, Joerg
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2022; Nature Publishing Group
• Subjects: 631/1647/245/2225; 631/57/2265; Binding; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brief Communication; Chemical compounds; Color; Deoxyribonucleic acid; DNA; DNA probes; Docking; Fluorescent Dyes; Fluorophores; Image acquisition; Image resolution; Kinetics; Life Sciences; Microscopes; Microscopy, Fluorescence - methods; Optical sectioning; Probes; Proteomics; Quenching; Sectioning; Single-stranded DNA

DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and slow imaging speed, both of which can be attributed to the prese...