Fast-atom Bombardment Mass Spectrometry for Mapping of Endogenous Methylated Purine Bases in Urine Extracts

• Published in: Rapid communications in mass spectrometry Vol. 11; no. 4; pp. 398 - 404
• Main Authors: Porcelli, Brunetta, Muraca, Lucia Filomena, Frosi, Barbara, Marinello, Enrico, Vernillo, Remo, De Martino, Antonio, Catinella, Silvia, Traldi, Pietro
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 28.02.1997
• Subjects: Colorectal Neoplasms - urine; Humans; Methylation; Nucleotide Mapping - instrumentation; Nucleotide Mapping - methods; Purines - urine; Spectrometry, Mass, Fast Atom Bombardment

Fast‐atom bombardment (FAB) mass spectrometry, linked with tandem mass spectrometry (MS/MS), was employed for the identification of methylated purine bases in four urinary extracts of healthy subjects and fourteen urinary extracts of patients bearing colorectal tumors. In order to obtain an easy str...