Influence of different glycosidic linkages on relative ion intensities in post-source decay fragmentation of a xyloglucan heptaoligosaccharide using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

• Published in: Rapid communications in mass spectrometry Vol. 12; no. 6; pp. 307 - 311
• Main Authors: Yamagaki, Tohru, Mitsuishi, Yasushi, Nakanishi, Hiroshi
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 31.03.1998
• Subjects: Carbohydrate Sequence; Glycosides - chemistry; Molecular Sequence Data; Polysaccharides - analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0951-4198; 1097-0231
• DOI: 10.1002/(SICI)1097-0231(19980331)12:6<307::AID-RCM155>3.0.CO;2-C

Post‐source decay fragment analysis using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) has been applied to a highly branched xyloglucan heptasaccharide from tamarind seed. All fragment ions were produced by cleavage of the glycosidic linkages, including multi‐site cleavages. The relative intensities of fragment ions that originated from one‐site cleavages of the glycosidic linkages were much higher than those arising from two‐site cleavages of the same kind of glycosidic linkage, which were in turn higher than those from three‐site cleavages. The types of glycosidic linkages were an important factor which influenced the relative intensities of the MALDI‐PSD (post‐source decay) fragment ions. In the MALDI‐PSD fragment spectrum of the xyloglucan heptasaccharide, the relative intensities of the ions produced by the cleavage of an α1‐6 glycosidic linkage were much higher than those arising from cleavage of the β1‐4 glycosidic linkage. © 1998 John Wiley & Sons, Ltd.