Transcriptional Mutagenesis Induced by Uracil and 8-Oxoguanine in Escherichia coli

• Published in: Molecular cell Vol. 12; no. 4; pp. 959 - 970
• Main Authors: Brégeon, Damien, Doddridge, Zara A, You, Ho Jin, Weiss, Bernard, Doetsch, Paul W
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2003; Cell Press
• Subjects: Base Sequence - drug effects; Base Sequence - genetics; DNA Damage - genetics; DNA Repair - genetics; DNA-Directed RNA Polymerases - metabolism; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli Proteins - biosynthesis; Escherichia coli Proteins - genetics; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - genetics; Guanine - analogs & derivatives; Guanine - pharmacology; Life Sciences; Mutagenesis - drug effects; Mutagenesis - genetics; Phenotype; Transcription, Genetic - drug effects; Transcription, Genetic - genetics; Uracil - metabolism; Uracil - pharmacology

Cells exposed to DNA damaging agents in their natural environment do not undergo continuous cycles of replication but are more frequently engaged in gene transcription. Luciferase gene expression analysis with DNA templates containing uracil or 8-oxoguanine, placed at a defined position, indicated t...