Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens

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Published inJournal of Clinical Microbiology Vol. 50; no. 5; pp. 1755 - 1757
Main Authors Alonso, María, Herranz, Marta, Martínez Lirola, Miguel, González-Rivera, Milagros, Bouza, Emilio, García de Viedma, Darío
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.05.2012
Subjects
Biological and medical sciences
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Minisatellite Repeats
Molecular Epidemiology
Molecular Typing
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Sputum - microbiology
Tuberculosis - epidemiology
Tuberculosis - microbiology
Molecular epidemiology
Smear
Genotype
Typing
Real time
Clinical isolate
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Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
ABSTRACT We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
Author Emilio Bouza
Marta Herranz
Milagros González-Rivera
Darío García de Viedma
María Alonso
Miguel Martínez Lirola
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10.1128/JCM.01392-06
10.1128/JCM.39.10.3563-3571.2001
10.1016/S0213-005X(11)70012-X
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Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61...
ABSTRACT We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61...
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61...
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SubjectTerms Biological and medical sciences
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Minisatellite Repeats
Molecular Epidemiology
Molecular Typing
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Sputum - microbiology
Tuberculosis - epidemiology
Tuberculosis - microbiology
Title Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens
URI http://jcm.asm.org/content/50/5/1755.abstract
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