Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens
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Published in | Journal of Clinical Microbiology Vol. 50; no. 5; pp. 1755 - 1757 |
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American Society for Microbiology
01.05.2012
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AbstractList | We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs. We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs. Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology. For an alternate route to JCM .asm.org, visit: JCM ABSTRACT We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs. |
Author | Emilio Bouza Marta Herranz Milagros González-Rivera Darío García de Viedma María Alonso Miguel Martínez Lirola |
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References | 11574573 - J Clin Microbiol. 2001 Oct;39(10):3563-71 16562710 - Int J Tuberc Lung Dis. 2006 Mar;10(3):297-304 17192416 - J Clin Microbiol. 2007 Mar;45(3):691-7 21420561 - Enferm Infecc Microbiol Clin. 2011 Mar;29 Suppl 1:8-13 18484876 - Clin Infect Dis. 2008 Jul 1;47(1):8-14 17005759 - J Clin Microbiol. 2006 Dec;44(12):4498-510 e_1_3_2_8_2 e_1_3_2_7_2 e_1_3_2_6_2 Nolte F (e_1_3_2_5_2) 1995 e_1_3_2_4_2 e_1_3_2_3_2 Cattamanchi A (e_1_3_2_2_2) 2006; 10 |
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Mendeley... We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61... ABSTRACT We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61... We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit–variable-number tandem-repeat typing) to directly analyze the bacilli present in 61... |
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SubjectTerms | Biological and medical sciences DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Humans Microbiology Minisatellite Repeats Molecular Epidemiology Molecular Typing Mycobacteriology and Aerobic Actinomycetes Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Sputum - microbiology Tuberculosis - epidemiology Tuberculosis - microbiology |
Title | Real-Time Molecular Epidemiology of Tuberculosis by Direct Genotyping of Smear-Positive Clinical Specimens |
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