Functional assessment of lysosomal Rab7 and RILP with RNA interference and overexpression in Spodoptera frugiperda Sf9 cell lines

• Published in: STAR protocols Vol. 4; no. 4; p. 102646
• Main Authors: Cui, Gaofeng, Jiang, Zhiyan, Zhong, Guohua
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.12.2023; Elsevier
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Animals; Cell Biology; Cell Line; Lysosomes - metabolism; Microscopy; Molecular Biology; Molecular/Chemical Probes; Protocol; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; rab7 GTP-Binding Proteins; RNA Interference; Spodoptera - genetics; Spodoptera - metabolism; Molecular/Chemical Probes; Microscopy; Molecular Biology; Cell Biology

The interaction manner and biological function of Rab7 and its effector, Rab-interacting lysosomal protein (RILP), remain unclear in invertebrates. We provide a protocol for detecting the effects of Rab7 and RILP terminals on lysosome and autophagy in Spodoptera frugiperda Sf9 cells with overexpress...