A surgical orthotopic approach for studying the invasive progression of human bladder cancer

• Published in: Nature protocols Vol. 14; no. 3; pp. 738 - 755
• Main Authors: Lorenzatti Hiles, Guadalupe, Cates, Angelica L., El-Sawy, Layla, Day, Kathleen C., Broses, Luke J., Han, Amy L., Briggs, Hannah L., Emamdjomeh, Amir, Chou, Andrew, Abel, Ethan V., Liebert, Monica, Palmbos, Phillip L., Udager, Aaron M., Keller, Evan T., Day, Mark L.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2019; Nature Publishing Group
• Subjects: 631/1647/767/70; 631/67/589/1336; Analytical Chemistry; Animal models; Animals; Biological Techniques; Biomedical and Life Sciences; Bladder; Bladder cancer; Cancer; Catheterization; Computational Biology/Bioinformatics; Development and progression; Diagnosis; Disease Models, Animal; Disease Progression; Humans; Implantation; Invasiveness; Laparotomy; Life Sciences; Metastases; Methods; Mice, Inbred NOD; Mice, SCID; Microarrays; Muscles; Neoplasm Invasiveness; Organic Chemistry; Protocol; Surgical equipment; Tumors; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - surgery; Urothelial cancer; Xenograft Model Antitumor Assays - methods; United States

The invasion of bladder cancer into the sub-urothelial muscle and vasculature are key determinants leading to lethal metastatic progression. However, the molecular basis is poorly understood, partly because of the lack of uncomplicated and reliable models that recapitulate the biology of locally inv...