Age and gender leucocytes variances and references values generated using the standardized ONE‐Study protocol

• Published in: Cytometry. Part A Vol. 89; no. 6; pp. 543 - 564
• Main Authors: Kverneland, Anders H., Streitz, Mathias, Geissler, Edward, Hutchinson, James, Vogt, Katrin, Boës, David, Niemann, Nadja, Pedersen, Anders Elm, Schlickeiser, Stephan, Sawitzki, Birgit
• Format: Journal Article
• Language: English
• Published: United States 01.06.2016
• Subjects: adaptive immunity; Adult; Age Factors; Aged; Aged, 80 and over; aging; Antigens, CD - genetics; Antigens, CD - immunology; Cohort Studies; Female; Flow Cytometry - standards; Healthy Volunteers; Humans; Immunologic Memory; Immunophenotyping - standards; immunosenescence; innate immunity; Lymphocyte Subsets - classification; Lymphocyte Subsets - cytology; Lymphocyte Subsets - immunology; Male; Middle Aged; Reference Values; Sex Factors; standardization; standardization; immunosenescence; aging; innate immunity; adaptive immunity

Flow cytometry is now accepted as an ideal technology to reveal changes in immune cell composition and function. However, it is also an error‐prone and variable technology, which makes it difficult to reproduce findings across laboratories. We have recently developed a strategy to standardize whole...