3D morphometry of red blood cells by digital holography

• Published in: Cytometry. Part A Vol. 85; no. 12; pp. 1030 - 1036
• Main Authors: Memmolo, Pasquale, Miccio, Lisa, Merola, Francesco, Gennari, Oriella, Netti, Paolo Antonio, Ferraro, Pietro
• Format: Journal Article
• Language: English
• Published: United States 01.12.2014
• Subjects: Algorithms; digital holography; Erythrocytes - cytology; Holography - methods; Humans; microfluidics; microscopy; Models, Theoretical; morphological analysis; optical tweezers; red blood cells; red blood cells; optical tweezers; microscopy; digital holography; microfluidics; morphological analysis

Three dimensional (3D) morphometric analysis of flowing and not‐adherent cells is an important aspect for diagnostic purposes. However, diagnostics tools need to be quantitative, label‐free and, as much as possible, accurate. Recently, a simple holographic approach, based on shape from silhouette al...