The synthesis of Rhodobacter capsulatus HupSL hydrogenase is regulated by the two‐component HupT/HupR system

• Published in: Molecular microbiology Vol. 34; no. 5; pp. 995 - 1006
• Main Authors: Dischert, Wanda, Vignais, Paulette M., Colbeau, Annette
• Format: Journal Article
• Language: English
• Published: Oxford BSL Blackwell Science Ltd 01.12.1999; Blackwell Publishing Ltd
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Blotting, Western; DNA Footprinting; DNA-Binding Proteins; DNA-Directed RNA Polymerases - metabolism; Gene Expression Regulation, Bacterial; hupR gene; HupR protein; hupSL gene; HupSL protein; HupT protein; Hydrogenase - biosynthesis; Hydrogenase - genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphorylation; Plasmids - genetics; Promoter Regions, Genetic; Rhodobacter capsulatus; Rhodobacter capsulatus - enzymology; Rhodobacter capsulatus - genetics; Sigma Factor - metabolism; Signal Transduction; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic

The synthesis of the membrane‐bound [NiFe]hydrogenase of Rhodobacter capsulatus (HupSL) is regulated negatively by the protein histidine kinase, HupT, and positively by the response regulator, HupR. It is demonstrated in this work that HupT and HupR are partners in a two‐component signal transductio...