Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo

• Published in: Marine drugs Vol. 16; no. 12; p. 473
• Main Authors: Zhang, Shan-Shan, Han, Li-Wen, Shi, Yong-Ping, Li, Xiao-Bin, Zhang, Xuan-Ming, Hou, Hai-Rong, Lin, Hou-Wen, Liu, Ke-Chun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.11.2018; MDPI
• Subjects: ACE-inhibitory activity; Amino acids; Angiotensin; anti-diabetic activity; Antidiabetics; antioxidant activity; Antioxidants; Apoptosis; Bio-assays; Bioassays; Biocompatibility; Biological activity; Diabetes; Diabetes mellitus; Digestive system; Freshwater fishes; Gastrointestinal tract; Hydrophobicity; Liquid chromatography; Low molecular weights; Mass spectrometry; Mass spectroscopy; Meat; Molecular docking; Molecular weight; Mollusks; multi-functional peptides; Neptunea; Neptunea arthritica cumingii; Peptides; Peptidyl-dipeptidase A; Protected species; Reactive oxygen species; Scavenging; Skin; Stability; Stability analysis; Synthetic peptides; Thermal simulation; Thermal stress; Toxicity; Zebrafish; Neptunea arthritica cumingii; ACE-inhibitory activity; anti-diabetic activity; antioxidant activity; multi-functional peptides
• DOI: 10.3390/md16120473
• ISSN: 1660-3397

Neptunea arthritica cumingii (Nac) is a marine snail with high nutritional and commercial value; however, little is known about its active peptides. In this study, two multi-functional peptides, YSQLENEFDR (Tyr-Ser-Gln-Leu-Glu-Asn-Glu-Phe-Asp-Arg) and YIAEDAER (Tyr-Ile-Ala-Glu-Asp-Ala-Glu-Arg), were isolated and purified from meat and visceral mass extracts of Nac using a multi-bioassay-guided method and were characterized by using liquid chromatography-tandem mass spectrometry. Both peptides showed high antioxidant, angiotensin-converting enzyme (ACE)-inhibitory, and anti-diabetic activities, with half-maximal effective concentrations values less than 1 mM. Antioxidant and ACE-inhibitory activities were significantly higher for YSQLENEFDR than for YIAEDAER. In a zebrafish model, the two peptides exhibited strong scavenging ability for reactive oxygen species and effectively protected skin cells against oxidative damage without toxicity. Molecular docking simulation further predicted the interactions of the two peptides and ACE. Stability analysis study indicated that the two synthetic peptides maintained their activities under thermal stress and simulated gastrointestinal digestion conditions. The low molecular weight, high proportion of hydrophobic and negatively-charged amino acids, and specific C-terminal and N-terminal amino acids may contribute to the observed bio-activities of these two peptides with potential application for the prevention of chronic noncommunicable diseases.