Mean platelet volume as an indicator of platelet activation: methodological issues

• Published in: Platelets (Edinburgh) Vol. 13; no. 5-6; pp. 301 - 306
• Main Authors: Park, Yongsoon, Schoene, Norberta, Harris, William
• Format: Journal Article
• Language: English
• Published: England Informa UK Ltd 2002; Taylor & Francis
• Subjects: Adult; Blood Platelets - cytology; Blood Platelets - physiology; Cell Size; Collagen - pharmacology; Fasting; Female; Humans; Hypertriglyceridemia - blood; Male; Middle Aged; Plant Oils - administration & dosage; Plant Oils - pharmacology; Platelet Activation; Platelet Function Tests - methods; Temperature
• ISSN: 0953-7104; 1369-1635
• DOI: 10.1080/095371002220148332

Background: Mean platelet volume (MPV) is increased in patients at high risk for athero-thrombotic diseases. Thus, an elevated MPV may be a risk marker for platelet activation. Methods: Healthy subjects with normal triglyceride (TG) levels (90 - 6 mg/dl; n = 40) or mild hypertriglyceridemia (161 - 79 mg/dl; n = 32) were studied. MPV was measured in fasting blood samples before and after stimulation with collagen (10 w g/ml), and exposure to 4 or 37°C. Samples from the normotriglyceridemic subjects were tested again 4 h after consuming a high-fat drink. Results: Collagen and exposure to 4°C increased MPV, whereas incubation at 37°C lowered MPV regardless of TG level. There was no significant difference in unstimulated MPV between the fasting and the fed states in the normotriglyceridemic subjects (both 7.2 - 0.1 fl; mean - SEM), nor between the latter group and hypertriglyceridemic subjects (7.0 - 0.1 fl). There was a significant negative relation between MPV and fasting TG level. Conclusions: This study suggests that MPV response to low-dose collagen may be a useful indicator of platelet propensity to activation. Further studies are warranted to correlate MPV with classical platelet aggregation tests and with the use of platelet-active drugs.