Characterization of the Mitogen-activated Protein Kinase Phosphorylation Sites on the Connexin-43 Gap Junction Protein (∗)

• Published in: The Journal of biological chemistry Vol. 271; no. 7; pp. 3779 - 3786
• Main Authors: Warn-Cramer, Bonnie J., Lampe, Paul D., Kurata, Wendy E., Kanemitsu, Martha Y., Loo, Lenora W.M., Eckhart, Walter, Lau, Alan F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.02.1996; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Cell Line; Connexin 43 - chemistry; Connexin 43 - metabolism; DNA Primers; Electrophoresis, Gel, Two-Dimensional; Epithelium - metabolism; Glutathione Transferase; Liver - metabolism; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Mapping; Phosphopeptides - chemistry; Phosphopeptides - isolation & purification; Phosphorylation; Polymerase Chain Reaction; Rats; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - metabolism; Spodoptera; Transfection; Trypsin

We have previously demonstrated that epidermal growth factor induced a rapid, transient decrease in gap junctional communication and increase in serine phosphorylation on the connexin-43 gap junction protein in T51B rat liver epithelial cells. The kinase(s) responsible for phosphorylation and specif...