Orthotopic and heterotopic murine models of pancreatic cancer and their different responses to FOLFIRINOX chemotherapy

• Published in: Disease models & mechanisms Vol. 11; no. 7
• Main Authors: Erstad, Derek J, Sojoodi, Mozhdeh, Taylor, Martin S, Ghoshal, Sarani, Razavi, Allen A, Graham-O'Regan, Katherine A, Bardeesy, Nabeel, Ferrone, Cristina R, Lanuti, Michael, Caravan, Peter, Tanabe, Kenneth K, Fuchs, Bryan C
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Ltd 01.07.2018; The Company of Biologists
• Subjects: Abdomen; Adenocarcinoma - drug therapy; Adenocarcinoma - pathology; Adenocarcinoma - surgery; Allografts; Animals; Antineoplastic Combined Chemotherapy Protocols - administration & dosage; Antineoplastic Combined Chemotherapy Protocols - pharmacology; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Autografts; Cancer therapies; Carcinoma, Pancreatic Ductal - drug therapy; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Pancreatic Ductal - surgery; Cell Line, Tumor; Cell Proliferation - drug effects; Chemoresistance; Disease Models, Animal; Drug Combinations; Fluorouracil - administration & dosage; Fluorouracil - pharmacology; Fluorouracil - therapeutic use; Heterotopic; Humans; Immune system; Immunotherapy; Kinetics; Laparotomy; Leucovorin - administration & dosage; Leucovorin - pharmacology; Leucovorin - therapeutic use; Male; Metastasis; Mice, Inbred C57BL; Mutation; Organometallic Compounds - administration & dosage; Organometallic Compounds - pharmacology; Organometallic Compounds - therapeutic use; Orthotopic; Pancreatic cancer; Pancreatic Neoplasms - blood supply; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - surgery; PDAC animal model; Phenotype; Spleen; Surgical technique; Surgical techniques; Transplantation, Heterotopic; Tumors; Heterotopic; Orthotopic; Surgical technique; Chemoresistance; PDAC animal model

Syngeneic, immunocompetent allograft tumor models recapitulate important aspects of the tumor microenvironment and have short tumor latency with predictable growth kinetics, making them useful for trialing novel therapeutics. Here, we describe surgical techniques for orthotopic and heterotopic pancr...