Molecular Detection of New SHV β-lactamase Variants in Clinical Escherichia coli and Klebsiella pneumoniae Isolates from Egypt

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 60; pp. 35 - 41
• Main Authors: Elmowalid, Gamal A., Ahmad, Adel Attia M., Hassan, Muhammad N., Abd El-Aziz, Norhan K., Abdelwahab, Ashraf M., Elwan, Shymaa I.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2018; Elsevier Science Ltd
• Subjects: Alleles; Amino acid sequence; amino acid sequences; amino acid substitution; Amino acids; Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Bacteria; beta-lactamase; beta-Lactamases - genetics; beta-lactams; Binding Sites; blaSHV alleles; blaSHV binding sites; broiler chickens; Chickens - microbiology; Chicks; Drug Resistance, Multiple, Bacterial - genetics; E coli; Egypt; Egypt - epidemiology; epidemiological studies; Epidemiology; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Infections - epidemiology; Gene transfer; Genetic relationship; genetic relationships; Humans; Juveniles; Klebsiella; Klebsiella Infections - epidemiology; Klebsiella pneumoniae; Klebsiella pneumoniae - enzymology; Klebsiella pneumoniae - genetics; MDR E. coli; MDR K. pneumoniae; Microbial Sensitivity Tests; Molecules; Multidrug resistance; multiple drug resistance; Mutation; pathogens; Phylogenetics; Phylogeny; Point Mutation; Proteins; sequence analysis; SHV-ESBL; Structure-function relationships; β-Lactam antibiotics; Egypt; MDR E. coli; SHV-ESBL; MDR K. pneumoniae; blaSHV alleles; blaSHV binding sites

•SHV-ESBL was detected in E. coli and K. peumoniae clinical isolates.•blaSHV-10, blaSHV-18, blaSHV-58 and blaSHV-91 were the most predominant variants.•New point mutations were detected in blaSHV and altered blaSHV enzyme binding sites.•blaSHV gene co-transfer was determined among isolates from huma...