Molecular characterization and identification of the E2/P4 response element in the porcine HOXA10 gene
Homeobox A10 ( HOXA10 ), a well-known transcriptional factor that can be regulated by estrogen (E 2 ) and progesterone (P 4 ), is necessary not only for endometrial differentiation but also for establishing the conditions required for implantation. However, little research has focused on the regulat...
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Published in | Molecular and cellular biochemistry Vol. 374; no. 1-2; pp. 213 - 222 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.02.2013
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Homeobox A10 (
HOXA10
), a well-known transcriptional factor that can be regulated by estrogen (E
2
) and progesterone (P
4
), is necessary not only for endometrial differentiation but also for establishing the conditions required for implantation. However, little research has focused on the regulation of the porcine
HOXA10
gene. In this study, we aimed to (1) characterize the genetic structure of the porcine
HOXA10
gene, (2) analyze the tissue expression pattern and differential expression levels in the endometrium of
HOXA10
in different developmental stages of Meishan and commercial Yorkshire pigs, and (3) identify the E2/P4 response element in the promoter region of the porcine
HOXA10
gene and verify that it induces
HOXA10
in human endometrial adenocarcinoma (Ishikawa) cells. The results indicated that the cDNA of porcine
HOXA10
was 2,628 bp in length with an open reading frame (ORF) of 1,236 bp encoding a peptide of 411-amino acid residues, which showed 90 and 95 % sequence similarity to that of human and mouse homologs, respectively. Semiquantitative RT-PCR confirmed that the porcine
HOXA10
gene is highly expressed in the endometrium, bladder and kidney. Real-time PCR showed that the expression of
HOXA10
was significantly higher on day 15 of gestation (gd 15) in comparison to gd 26 (
P
< 0.01), gd 50 (
P
< 0.01) and day 15 of the estrous cycle (ed 15) in the endometrium of Meishan pigs. In contrast, the highest expression in the endometrium of Yorkshire pigs was on gd 50. Moreover, the abundance of
HOXA10
mRNA was the highest on gd 15 in Meishan pigs than in any other stage tested in the two breeds. Deletion analysis and reporter expression assays identified a promoter region (−1044 bp to +18 bp) which is responsible for E
2
- and P
4
-induced
HOXA10
transcription. Moreover, this promoter region enhanced the E2/P4-induced
HOXA10
expression in Ishikawa cells. In conclusion, we identified an E
2
/P
4
response element of the porcine
HOXA10
gene for the first time. The data from the present study contribute to the mechanism by which the porcine
HOXA10
gene regulates embryo implantation and prolificacy traits. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0300-8177 1573-4919 |
DOI: | 10.1007/s11010-012-1522-5 |