Epithelial-mesenchymal transition markers in pancreatic ductal adenocarcinoma
Expression of transcription factors that mediate epithelial-mesenchymal transition (EMT), such as Twist and Slug, is correlated with poor prognosis in many tumor types. Selected EMT markers were studied in a series of pancreatic ductal adenocarcinomas (PDAs) and benign pancreatic tissues to determin...
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Published in | Pancreas Vol. 38; no. 1; p. e1 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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01.01.2009
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Abstract | Expression of transcription factors that mediate epithelial-mesenchymal transition (EMT), such as Twist and Slug, is correlated with poor prognosis in many tumor types. Selected EMT markers were studied in a series of pancreatic ductal adenocarcinomas (PDAs) and benign pancreatic tissues to determine whether expression levels correlated with diagnosis, histologic grade, or patient outcome.
Immunohistochemical stains for Twist, Slug, and N-cadherin were performed using a tissue microarray containing 68 PDAs and 38 samples of normal pancreas or chronic pancreatitis tissues.
Twist and Slug were identified in both the nucleus and cytoplasm of benign pancreatic ductal epithelium, chronic pancreatitis, and PDA. Compared with normal ductal epithelium, nuclear levels of Twist are decreased in PDA. None of the other EMT markers showed significant differences in staining indices among the diagnostic groups. There were no correlations between EMT marker expression and histologic grade. Epithelial-mesenchymal transition marker expression was not associated with N-cadherin expression, patient outcome, or duration of survival.
Decreased expression of nuclear Twist is observed in malignant pancreatic epithelium. However, use of Twist as a diagnostic marker is precluded because decreased expression is also seen in chronic pancreatitis. None of the markers studied were predictive of patient outcome. |
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AbstractList | Expression of transcription factors that mediate epithelial-mesenchymal transition (EMT), such as Twist and Slug, is correlated with poor prognosis in many tumor types. Selected EMT markers were studied in a series of pancreatic ductal adenocarcinomas (PDAs) and benign pancreatic tissues to determine whether expression levels correlated with diagnosis, histologic grade, or patient outcome.
Immunohistochemical stains for Twist, Slug, and N-cadherin were performed using a tissue microarray containing 68 PDAs and 38 samples of normal pancreas or chronic pancreatitis tissues.
Twist and Slug were identified in both the nucleus and cytoplasm of benign pancreatic ductal epithelium, chronic pancreatitis, and PDA. Compared with normal ductal epithelium, nuclear levels of Twist are decreased in PDA. None of the other EMT markers showed significant differences in staining indices among the diagnostic groups. There were no correlations between EMT marker expression and histologic grade. Epithelial-mesenchymal transition marker expression was not associated with N-cadherin expression, patient outcome, or duration of survival.
Decreased expression of nuclear Twist is observed in malignant pancreatic epithelium. However, use of Twist as a diagnostic marker is precluded because decreased expression is also seen in chronic pancreatitis. None of the markers studied were predictive of patient outcome. |
Author | Byrd, Robert H Cates, Justin M M Fohn, Laurel E Washington, Mary K Black, Candice C Tatsas, Armanda D |
Author_xml | – sequence: 1 givenname: Justin M M surname: Cates fullname: Cates, Justin M M email: Justin.M.Cates@Vanderbilt.edu organization: Department of Pathology, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Justin.M.Cates@Vanderbilt.edu – sequence: 2 givenname: Robert H surname: Byrd fullname: Byrd, Robert H – sequence: 3 givenname: Laurel E surname: Fohn fullname: Fohn, Laurel E – sequence: 4 givenname: Armanda D surname: Tatsas fullname: Tatsas, Armanda D – sequence: 5 givenname: Mary K surname: Washington fullname: Washington, Mary K – sequence: 6 givenname: Candice C surname: Black fullname: Black, Candice C |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/18766116$$D View this record in MEDLINE/PubMed |
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References | 17699854 - Clin Cancer Res. 2007 Aug 15;13(16):4769-76 16707493 - J Biol Chem. 2006 Jul 28;281(30):21321-31 15208362 - J Histochem Cytochem. 2004 Jul;52(7):959-65 17243120 - J Cell Biochem. 2007 Jul 1;101(4):816-29 15217949 - Clin Cancer Res. 2004 Jun 15;10(12 Pt 1):4125-33 16024596 - Cancer Res. 2005 Jul 15;65(14):5996-6000; discussion 6000-1 16169465 - Cancer Cell. 2005 Sep;8(3):197-209 16564917 - Hum Pathol. 2006 Apr;37(4):431-8 15790452 - Gynecol Oncol. 2005 Apr;97(1):155-65 12082640 - Oncogene. 2002 May 9;21(20):3241-6 15709186 - Clin Cancer Res. 2005 Feb 1;11(3):1174-80 18301244 - Appl Immunohistochem Mol Morphol. 2008 May;16(3):251-62 12632084 - Int J Oncol. 2003 Apr;22(4):891-8 17394502 - Histopathology. 2007 Apr;50(5):648-58 16567498 - J Cell Biol. 2006 Mar 27;172(7):973-81 16299238 - Clin Cancer Res. 2005 Nov 15;11(22):8070-8 16822877 - J Clin Pathol. 2007 May;60(5):510-4 15742334 - Cancer. 2005 Apr 15;103(8):1631-43 17211477 - Br J Cancer. 2007 Jan 29;96(2):314-20 17299729 - J Pathol. 2007 Apr;211(5):507-15 11912130 - Cancer Res. 2002 Mar 15;62(6):1613-8 16002843 - J Clin Oncol. 2005 Jul 10;23(20):4524-31 16773075 - Br J Cancer. 2006 Jun 19;94(12):1816-22 17512904 - Biochem Biophys Res Commun. 2007 Jul 6;358(3):925-30 15644775 - Am J Surg Pathol. 2005 Feb;29(2):188-95 17583745 - J Surg Res. 2007 Aug;141(2):196-203 15983400 - Development. 2005 Jul;132(14):3151-61 16585154 - Cancer Res. 2006 Apr 1;66(7):3365-9 15864483 - Ann Surg Oncol. 2005 Jun;12(6):488-96 17236203 - Int J Cancer. 2007 Apr 15;120(8):1634-40 11054563 - Gene. 2000 Oct 17;257(1):1-12 16024595 - Cancer Res. 2005 Jul 15;65(14):5991-5; discussion 5995 12414534 - Am J Pathol. 2002 Nov;161(5):1881-91 17674958 - Semin Oncol. 2007 Aug;34(4):303-10 14644200 - Curr Opin Cell Biol. 2003 Dec;15(6):740-6 15958559 - Cancer Res. 2005 Jun 15;65(12):5153-62 17258791 - Hum Pathol. 2007 Apr;38(4):598-606 17558857 - Pathology. 2007 Jun;39(3):305-18 15210113 - Cell. 2004 Jun 25;117(7):927-39 8423624 - J Natl Cancer Inst. 1993 Feb 3;85(3):200-6 |
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SubjectTerms | Adult Aged Aged, 80 and over Antigens, CD - analysis Biomarkers, Tumor - analysis Cadherins - analysis Carcinoma, Pancreatic Ductal - chemistry Carcinoma, Pancreatic Ductal - mortality Carcinoma, Pancreatic Ductal - pathology Cell Transdifferentiation Down-Regulation Epithelial Cells - chemistry Epithelial Cells - pathology Humans Immunohistochemistry Mesoderm - chemistry Mesoderm - pathology Middle Aged Neoplasm Staging Nuclear Proteins - analysis Pancreatic Neoplasms - chemistry Pancreatic Neoplasms - mortality Pancreatic Neoplasms - pathology Pancreatitis, Chronic - metabolism Pancreatitis, Chronic - pathology Prognosis Snail Family Transcription Factors Tissue Array Analysis Transcription Factors - analysis Twist-Related Protein 1 - analysis |
Title | Epithelial-mesenchymal transition markers in pancreatic ductal adenocarcinoma |
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