Evaluation of Immortalized AVPV- and Arcuate-Specific Neuronal Kisspeptin Cell Lines to Elucidate Potential Mechanisms of Estrogen Responsiveness and Temporal Gene Expression in Females

• Published in: Endocrinology (Philadelphia) Vol. 157; no. 9; pp. 3410 - 3419
• Main Authors: Jacobs, Dakota C, Veitch, Rebecca E, Chappell, Patrick E
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01.09.2016
• Subjects: Animals; Arcuate Nucleus of Hypothalamus - cytology; Arcuate Nucleus of Hypothalamus - metabolism; Cell Line - metabolism; Circadian Rhythm; Estradiol - metabolism; Female; Gene Expression Regulation; Hypothalamus, Anterior - cytology; Hypothalamus, Anterior - metabolism; Kisspeptins - metabolism; Kisspeptins - secretion; Mice, Transgenic; Neurons - metabolism

In females, ovarian estradiol modulates kisspeptin (Kiss-1) synthesis to act as an obligatory regulator of downstream gonadotropin release in vivo, via stimulation of GnRH neurons. Changes in the ovarian condition are relayed to the neuroendocrine hypothalamus via two sexually dimorphic Kiss-1 popul...