Oxidative phosphorylation and respiratory control phenomenon in Paracoccus denitrificans plasma membrane

• Published in: Biochemistry (Moscow) Vol. 77; no. 9; pp. 1000 - 1007
• Main Authors: Zharova, T. V., Vinogradov, A. D.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.09.2012; Springer; Springer Nature B.V
• Subjects: Adenosine triphosphatase; Adenosine Triphosphate - biosynthesis; Animals; Biocatalysis; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cell Membrane - enzymology; Cell Membrane - metabolism; Cell membranes; Electron Transport; Kinetics; Life Sciences; Microbiology; Mitochondrial DNA; Mitochondrial Proton-Translocating ATPases - metabolism; Oxidation-Reduction; Oxidative Phosphorylation; Paracoccus denitrificans; Paracoccus denitrificans - cytology; Paracoccus denitrificans - metabolism; Permeability; Phosphorylation; Plasma; Rats; Respiratory system; bacterial plasma membrane; H; ATP synthase; F; oxidative phosphorylation
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297912090064

Changes in respiratory activity, transmembrane electric potential, and ATP synthesis as induced by additions of limited amounts of ADP and P i to tightly coupled inverted (inside-out) Paracoccus denitrificans plasma membrane vesicles were traced. The pattern of the changes was qualitatively the same as those observed for coupled mitochondria during the classical State 4-State 3-State 4 transition. Bacterial vesicles devoid of energy-dependent permeability barriers for the substrates of oxidation and phosphorylation were used as a simple experimental model to investigate two possible mechanisms of respiratory control: (i) in State 4 phosphoryl transfer potential (ATP/ADP × P i ) is equilibrated with proton-motive force by reversibly operating F 1 ·F o -ATPase (thermodynamic control); (ii) in State 4 apparent “equilibrium” is reached by unidirectional operation of proton motive force-activated F 1 ·F o -ATP synthase. The data support the kinetic mechanism of the respiratory control phenomenon.