An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells

Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We...

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Published inMicromachines (Basel) Vol. 12; no. 5; p. 473
Main Authors Jou, Hei-Jen, Chou, Li-Yun, Chang, Wen-Chun, Ho, Hsin-Cheng, Zhang, Wan-Ting, Ling, Pei-Ying, Tsai, Ko-Hsin, Chen, Szu-Hua, Chen, Tze-Ho, Lo, Pei-Hsuan, Chen, Ming, Hsu, Heng-Tung
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 21.04.2021
MDPI
Subjects
Antibodies
Automation
Biomarkers
Biopsy
Cancer
CD13
circulating tumor cells
Efficiency
epithelial ovarian cancer
liquid biopsy
Metastasis
Microfluidics
Ovarian cancer
Polyethylene glycol
Sensitivity
SKOV3
Tumors
United States > US
Taiwan
SKOV3
epithelial ovarian cancer
CD13
circulating tumor cells
liquid biopsy
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Abstract Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We have designed an automated microfluidic chip-based CTC detection system and hypothesize this novel system can reliably detect CTC from clinical specimens. SKOV3 ovarian cancer cell line was used first to test the reliability of our system. Ten healthy volunteers, 5 patients with benign ovarian tumors, and 8 patients with epithelial ovarian cancer (EOC) were recruited to validate the CTC capturing efficacy in the peripheral blood. The capture rates for spiking test in SKOV3 cells were 48.3% and 89.6% by using anti-EpCAM antibody alone and a combination of anti-EpCAM antibody and anti-N-cadherin antibody, respectively. The system was sensitive to detection of low cell count and showed a linear relationship with the cell counts in our test range. The sensitivity and specificity were 62.5% and 100% when CTC was used as a biomarker for EOC. Our results demonstrated that this automatic CTC platform has a high capture rate and is feasible for detection of CTCs in EOC.
AbstractList Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We have designed an automated microfluidic chip-based CTC detection system and hypothesize this novel system can reliably detect CTC from clinical specimens. SKOV3 ovarian cancer cell line was used first to test the reliability of our system. Ten healthy volunteers, 5 patients with benign ovarian tumors, and 8 patients with epithelial ovarian cancer (EOC) were recruited to validate the CTC capturing efficacy in the peripheral blood. The capture rates for spiking test in SKOV3 cells were 48.3% and 89.6% by using anti-EpCAM antibody alone and a combination of anti-EpCAM antibody and anti-N-cadherin antibody, respectively. The system was sensitive to detection of low cell count and showed a linear relationship with the cell counts in our test range. The sensitivity and specificity were 62.5% and 100% when CTC was used as a biomarker for EOC. Our results demonstrated that this automatic CTC platform has a high capture rate and is feasible for detection of CTCs in EOC.Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We have designed an automated microfluidic chip-based CTC detection system and hypothesize this novel system can reliably detect CTC from clinical specimens. SKOV3 ovarian cancer cell line was used first to test the reliability of our system. Ten healthy volunteers, 5 patients with benign ovarian tumors, and 8 patients with epithelial ovarian cancer (EOC) were recruited to validate the CTC capturing efficacy in the peripheral blood. The capture rates for spiking test in SKOV3 cells were 48.3% and 89.6% by using anti-EpCAM antibody alone and a combination of anti-EpCAM antibody and anti-N-cadherin antibody, respectively. The system was sensitive to detection of low cell count and showed a linear relationship with the cell counts in our test range. The sensitivity and specificity were 62.5% and 100% when CTC was used as a biomarker for EOC. Our results demonstrated that this automatic CTC platform has a high capture rate and is feasible for detection of CTCs in EOC.
Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with the CTC detection have limited its potential impact on clinical application. A reliable automated CTC detection system is therefore needed. We have designed an automated microfluidic chip-based CTC detection system and hypothesize this novel system can reliably detect CTC from clinical specimens. SKOV3 ovarian cancer cell line was used first to test the reliability of our system. Ten healthy volunteers, 5 patients with benign ovarian tumors, and 8 patients with epithelial ovarian cancer (EOC) were recruited to validate the CTC capturing efficacy in the peripheral blood. The capture rates for spiking test in SKOV3 cells were 48.3% and 89.6% by using anti-EpCAM antibody alone and a combination of anti-EpCAM antibody and anti-N-cadherin antibody, respectively. The system was sensitive to detection of low cell count and showed a linear relationship with the cell counts in our test range. The sensitivity and specificity were 62.5% and 100% when CTC was used as a biomarker for EOC. Our results demonstrated that this automatic CTC platform has a high capture rate and is feasible for detection of CTCs in EOC.
Author Lo, Pei-Hsuan
Chen, Szu-Hua
Chen, Ming
Hsu, Heng-Tung
Zhang, Wan-Ting
Chen, Tze-Ho
Chang, Wen-Chun
Jou, Hei-Jen
Tsai, Ko-Hsin
Ling, Pei-Ying
Chou, Li-Yun
Ho, Hsin-Cheng
AuthorAffiliation 9 Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 526, Taiwan
6 Circulating Rare Cell Laboratory, Taiwan Adventist Hospital, Taipei 100, Taiwan; szu2520@gmail.com
1 Departments of Obstetrics and Gynecology, Taiwan Adventist Hospital, Taipei 105, Taiwan; peiyingling03@gmail.com (P.-Y.L.); 150188@tahsda.org.tw (K.-H.T.); pkmmcl@gmail.com (P.-H.L.)
3 International College of Semiconductor Technology, National Yang Ming Chiao Tung University, Hsinchu 30010, Taiwan; neoho.icst07g@nctu.edu.tw
4 School of Nursing, National Taipei University of Nursing and Health Science, Taipei 112, Taiwan
8 Department of Genomic Science and Technology, Changhua Christian Hospital Healthcare System, Changhua 526, Taiwan
7 Department of Genomic Medicine and Center for Medical Genetics, Changhua Christian Hospital, Changhua 526, Taiwan; 46305@cch.org.tw
2 Departments of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei 100, Taiwan; 143178@tahsda.org.tw (L.-Y.C.); dto
AuthorAffiliation_xml – name: 1 Departments of Obstetrics and Gynecology, Taiwan Adventist Hospital, Taipei 105, Taiwan; peiyingling03@gmail.com (P.-Y.L.); 150188@tahsda.org.tw (K.-H.T.); pkmmcl@gmail.com (P.-H.L.)
– name: 9 Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 526, Taiwan
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Keywords SKOV3
epithelial ovarian cancer
CD13
circulating tumor cells
liquid biopsy
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Snippet Circulating tumor cell (CTC) test is currently used as a biomarker in cancer treatment. Unfortunately, the poor reproducibility and limited sensitivity with...
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SubjectTerms Antibodies
Automation
Biomarkers
Biopsy
Cancer
CD13
circulating tumor cells
Efficiency
epithelial ovarian cancer
liquid biopsy
Metastasis
Microfluidics
Ovarian cancer
Polyethylene glycol
Sensitivity
SKOV3
Tumors
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Title An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells
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Volume 12
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