Whole-mount immunofluorescent labeling of the adult fly retina

• Published in: STAR protocols Vol. 3; no. 2; p. 101430
• Main Authors: Hsiao, Min-Chun, Huang, Ting-Yi, Yu, Bo-Hua, Chang, Tse-Chia, Chang, Hui-Yun, Sang, Tzu-Kang
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17.06.2022; Elsevier
• Subjects: Actins; Animals; Cell Biology; Drosophila; Histological Techniques - methods; Microscopy; Model Organisms; Protocol; Retina - diagnostic imaging; Microscopy; Model Organisms; Cell Biology

The adult Drosophila compound eye is an ideal in vivo model for studying biological questions. However, light microscopy of this tissue requires cumbersome embedding and sectioning. Here, we document detailed whole-mount procedures for immunolabeling the adult retina, enabling high-quality studies o...