DNA Sequencing from Subcritical Concentration of Cell-Free DNA Extracted from Electrowetting-on-Dielectric Platform

• Published in: Micromachines (Basel) Vol. 13; no. 4; p. 507
• Main Authors: Alias, Anand Baby, Huang, Hong-Yuan, Wang, Yi-Wen, Lin, Kai-Ti, Lu, Pei-Jhen, Wu, Tzu-Hui, Jiang, Pei-Shin, Chen, Chien-An, Yao, Da-Jeng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.03.2022; MDPI
• Subjects: Amplification; BLAST; cf-DNA extraction; DNA sequencing; Electrodes; Embryos; EWOD; Gene sequencing; Genetic testing; modified nested PCR; Nucleotides; Polymerase chain reaction; Quantitative analysis; Reagents; Wetting; Taiwan; cf-DNA extraction; modified nested PCR; BLAST; EWOD; DNA sequencing

Electro-Wetting-On-Dielectric (EWOD) based digital operations have demonstrated outstanding potential in actuating and manipulating liquid droplets. Here, we adapted the EWOD for extracting femtogram quantities of cell-free DNA (cf-DNA) from 1 μL of KSOM mouse embryo culture medium. Our group extrac...