Use of red-shifted dyes in a fluorescence polarization AKT kinase assay for detection of biological activity in natural product extracts
Kinases are an important therapeutic target for drug discovery, and many cancer chemotherapeutic agents have been derived from natural product sources. Natural product samples, however, have the likelihood of assay interference, particularly at elevated test concentrations. The authors developed a c...
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| Published in | Journal of biomolecular screening Vol. 9; no. 1; pp. 52 - 61 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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United States
01.02.2004
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| Subjects | |
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| Abstract | Kinases are an important therapeutic target for drug discovery, and many cancer chemotherapeutic agents have been derived from natural product sources. Natural product samples, however, have the likelihood of assay interference, particularly at elevated test concentrations. The authors developed a competitive fluorescence polarization (FP) assay using red-shifted fluorophores for the AKT kinase and demonstrated utility for testing concentrated natural product extracts. A set of 7 actinomycetes cultures containing indolocarbazoles, known nonselective kinase inhibitors, and a control set of 22 nonproducing indolocarbazole cultures were evaluated. Using red-shifted dyes (Cy3B or Cy5), the authors identified active samples with minimal interference up to the extract concentrations that are 3 times nonextracted culture levels. In contrast, a significant number of interferences were observed using either a fluorescein competitive FP assay or a [33P]ATP Flashplate assay. This work demonstrates that one can screen natural product extracts at high concentrations successfully using FP technology with red-shifted dyes. |
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| AbstractList | Kinases are an important therapeutic target for drug discovery, and many cancer chemotherapeutic agents have been derived from natural product sources. Natural product samples, however, have the likelihood of assay interference, particularly at elevated test concentrations. The authors developed a competitive fluorescence polarization (FP) assay using red-shifted fluorophores for the AKT kinase and demonstrated utility for testing concentrated natural product extracts. A set of 7 actinomycetes cultures containing indolocarbazoles, known nonselective kinase inhibitors, and a control set of 22 nonproducing indolocarbazole cultures were evaluated. Using red-shifted dyes (Cy3B or Cy5), the authors identified active samples with minimal interference up to the extract concentrations that are 3 times nonextracted culture levels. In contrast, a significant number of interferences were observed using either a fluorescein competitive FP assay or a [33P]ATP Flashplate assay. This work demonstrates that one can screen natural product extracts at high concentrations successfully using FP technology with red-shifted dyes. |
| Author | Horan, Ann C Hart, Richard F Turek-Etienne, Tammy C Langsdorf, Erik F Terracciano, Joseph S Bryant, Robert W Lei, Ming |
| Author_xml | – sequence: 1 givenname: Tammy C surname: Turek-Etienne fullname: Turek-Etienne, Tammy C email: tammy.turek-etienne@spcorp.com organization: Department of New Lead Discovery, Schering-Plough Research Institute, Kenilworth, NJ 07033-1300, USA. tammy.turek-etienne@spcorp.com – sequence: 2 givenname: Ming surname: Lei fullname: Lei, Ming – sequence: 3 givenname: Joseph S surname: Terracciano fullname: Terracciano, Joseph S – sequence: 4 givenname: Erik F surname: Langsdorf fullname: Langsdorf, Erik F – sequence: 5 givenname: Robert W surname: Bryant fullname: Bryant, Robert W – sequence: 6 givenname: Richard F surname: Hart fullname: Hart, Richard F – sequence: 7 givenname: Ann C surname: Horan fullname: Horan, Ann C |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/15006149$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1128/MCB.21.3.893-901.2001 10.1006/abio.2001.5412 10.1177/108705710000500204 10.1002/med.1022 10.1016/S1097-2765(01)00324-0 10.1006/abio.1997.2365 10.1358/dot.2002.38.9.696536 10.1021/ac025534s 10.1073/pnas.93.8.3636 10.1093/jnci/95.4.291 10.7164/antibiotics.42.1547 10.1016/S1359-6446(98)01204-5 10.1021/np9604893 10.1016/S1359-6446(01)01856-6 10.1177/108705710100600106 10.1006/excr.1999.4690 10.1021/np9800102 10.1101/gad.13.22.2905 10.1016/S1359-6446(00)01511-7 10.1177/108705710100600407 10.1177/108705710000500106 10.1177/1087057103252304 10.1016/S0304-4165(01)00143-X 10.1006/abio.1999.4029 10.1006/abio.1998.3030 10.1038/35077225 10.1074/jbc.274.31.21528 10.1016/S0092-8674(02)01083-8 10.1006/abio.1997.2455 10.1016/S0092-8674(00)80405-5 10.1177/108705710200700304 10.1038/nrc723 10.1002/(SICI)1521-3773(19990301)38:5<643::AID-ANIE643>3.0.CO;2-G 10.1177/108705710000500609 |
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| References | Datta (10.1177/1087057103259346_bib10) 1997; 91 Blume-Jensen (10.1177/1087057103259346_bib8) 2001; 411 Kim (10.1177/1087057103259346_bib11) 2001; 21 Harvey (10.1177/1087057103259346_bib23) 2000; 5 Hernandez-Boluda (10.1177/1087057103259346_bib4) 2002; 38 Shu (10.1177/1087057103259346_bib17) 1998; 61 Deshpande (10.1177/1087057103259346_bib26) 1999; 3603 McDonald (10.1177/1087057103259346_bib32) 1999; 268 Turek (10.1177/1087057103259346_bib29) 2001; 299 Sills (10.1177/1087057103259346_bib30) 2002; 7 10.1177/1087057103259346_bib20 Bindseil (10.1177/1087057103259346_bib21) 2001; 6 Seethala (10.1177/1087057103259346_bib24) 1997; 253 Brazil (10.1177/1087057103259346_bib6) 2002; 111 Datta (10.1177/1087057103259346_bib9) 1999; 13 Traxler (10.1177/1087057103259346_bib3) 2001; 21 Zimmermann (10.1177/1087057103259346_bib5) 2002; 56 Kolb (10.1177/1087057103259346_bib36) 1998; 3 Kumar (10.1177/1087057103259346_bib38) 2001; 1526 Bader (10.1177/1087057103259346_bib37) 2001; 6 Earnshaw (10.1177/1087057103259346_bib35) 2001; 6 Cheng (10.1177/1087057103259346_bib13) 1996; 93 Fabbro (10.1177/1087057103259346_bib1) 2002; 5 Orchard (10.1177/1087057103259346_bib2) 2002; 5 Kandel (10.1177/1087057103259346_bib7) 1999; 253 Matson (10.1177/1087057103259346_bib39) 1989; 42 Lee (10.1177/1087057103259346_bib12) 2001; 8 Mann (10.1177/1087057103259346_bib18) 2002; 2 Parker (10.1177/1087057103259346_bib28) 2000; 5 Park (10.1177/1087057103259346_bib33) 1999; 269 Henkel (10.1177/1087057103259346_bib19) 1999; 38 Wu (10.1177/1087057103259346_bib27) 2000; 5 Eldridge (10.1177/1087057103259346_bib22) 2002; 74 Seethala (10.1177/1087057103259346_bib25) 1998; 255 Ohmi (10.1177/1087057103259346_bib34) 2000; 5 Chun (10.1177/1087057103259346_bib14) 2003; 95 Turek-Etienne (10.1177/1087057103259346_bib31) 2003; 8 Cragg (10.1177/1087057103259346_bib16) 1997; 60 Nakatani (10.1177/1087057103259346_bib15) 1999; 274 |
| References_xml | – volume: 21 start-page: 893 year: 2001 ident: 10.1177/1087057103259346_bib11 article-title: AKT phosphorylates and negatively regulates apoptosis signal-regulating kinase 1 publication-title: Mol Cell Biol doi: 10.1128/MCB.21.3.893-901.2001 contributor: fullname: Kim – volume: 299 start-page: 45 year: 2001 ident: 10.1177/1087057103259346_bib29 article-title: Development and validation of a competitive AKT serine/threonine kinase fluorescence polarization assay using a product-specific anti-phospho-serine antibody publication-title: Anal Biochem doi: 10.1006/abio.2001.5412 contributor: fullname: Turek – volume: 5 start-page: 77 year: 2000 ident: 10.1177/1087057103259346_bib28 article-title: Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand-binding and kinase*/phosphatase assays publication-title: J Biomol Screen doi: 10.1177/108705710000500204 contributor: fullname: Parker – volume: 21 start-page: 499 year: 2001 ident: 10.1177/1087057103259346_bib3 article-title: Tyrosine kinase inhibitors: from rational design to clinical trials publication-title: Med Res Rev doi: 10.1002/med.1022 contributor: fullname: Traxler – volume: 8 start-page: 693 year: 2001 ident: 10.1177/1087057103259346_bib12 article-title: AKT-mediated phosphorylation of the G protein-coupled receptor EDG-1 is required for endothelial cell chemotaxis publication-title: Mol Cell doi: 10.1016/S1097-2765(01)00324-0 contributor: fullname: Lee – volume: 253 start-page: 210 year: 1997 ident: 10.1177/1087057103259346_bib24 article-title: A homogeneous, fluorescence polarization assay for src-family tyrosine kinases publication-title: Anal Biochem doi: 10.1006/abio.1997.2365 contributor: fullname: Seethala – volume: 38 start-page: 601 year: 2002 ident: 10.1177/1087057103259346_bib4 article-title: Imatinib mesylate (Gleeve/Glivec): a new therapy for chronic myeloid leukemia and other malignancies publication-title: Drugs Today doi: 10.1358/dot.2002.38.9.696536 contributor: fullname: Hernandez-Boluda – volume: 74 start-page: 3963 year: 2002 ident: 10.1177/1087057103259346_bib22 article-title: High-throughput method for the production and analysis of large natural product libraries for drug discovery publication-title: Anal Chem doi: 10.1021/ac025534s contributor: fullname: Eldridge – volume: 93 start-page: 3636 year: 1996 ident: 10.1177/1087057103259346_bib13 article-title: Amplification of AKT2 in human pancreatic cancer cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.93.8.3636 contributor: fullname: Cheng – volume: 95 start-page: 291 year: 2003 ident: 10.1177/1087057103259346_bib14 article-title: Effects of deguelin on the phosphatidylinositol 3-kinase/AKT pathway and apoptosis in premalignant human bronchial epithelial cells publication-title: J Natl Cancer Inst doi: 10.1093/jnci/95.4.291 contributor: fullname: Chun – ident: 10.1177/1087057103259346_bib20 – volume: 42 start-page: 1547 year: 1989 ident: 10.1177/1087057103259346_bib39 article-title: AT2433-A1, AT2433-A2, AT2433-B1, and AT2433-B2 novel antitumor anti-biotic compounds produced by Actinomadura melliaura: taxonomy, fermentation, isolation and biological properties publication-title: J Antibiot doi: 10.7164/antibiotics.42.1547 contributor: fullname: Matson – volume: 3 start-page: 333 year: 1998 ident: 10.1177/1087057103259346_bib36 article-title: Tyrosine kinase assays adapted to homogeneous time-resolved fluorescence publication-title: Drug Discov Today doi: 10.1016/S1359-6446(98)01204-5 contributor: fullname: Kolb – volume: 60 start-page: 52 year: 1997 ident: 10.1177/1087057103259346_bib16 article-title: Natural products in drug discovery and development publication-title: J Nat Prod doi: 10.1021/np9604893 contributor: fullname: Cragg – volume: 6 start-page: 840 year: 2001 ident: 10.1177/1087057103259346_bib21 article-title: Pure compound libraries: a new perspective for natural product based drug discovery publication-title: Drug Discov Today doi: 10.1016/S1359-6446(01)01856-6 contributor: fullname: Bindseil – volume: 5 start-page: 701 year: 2002 ident: 10.1177/1087057103259346_bib1 article-title: Targeting protein kinases in cancer therapy publication-title: Curr Opin Drug Discov Dev contributor: fullname: Fabbro – volume: 6 start-page: 39 year: 2001 ident: 10.1177/1087057103259346_bib35 article-title: FlashPlate scintillation proximity assays for characterization and screening of DNA polymerase, primase, and helicase activities publication-title: J Biomol Screen doi: 10.1177/108705710100600106 contributor: fullname: Earnshaw – volume: 253 start-page: 210 year: 1999 ident: 10.1177/1087057103259346_bib7 article-title: The regulation and activities of the multifunctional serine/threonine kinase AKT/PKB publication-title: Exp Cell Res doi: 10.1006/excr.1999.4690 contributor: fullname: Kandel – volume: 61 start-page: 1053 year: 1998 ident: 10.1177/1087057103259346_bib17 article-title: Recent natural products based drug development: a pharmaceutical industry perspective publication-title: J Nat Prod doi: 10.1021/np9800102 contributor: fullname: Shu – volume: 13 start-page: 2905 year: 1999 ident: 10.1177/1087057103259346_bib9 article-title: Cellular survival: a play in three AKTs publication-title: Genes Dev doi: 10.1101/gad.13.22.2905 contributor: fullname: Datta – volume: 5 start-page: 294 year: 2000 ident: 10.1177/1087057103259346_bib23 article-title: Strategies for discovering drugs from previously unexplored natural products publication-title: Drug Discov Today doi: 10.1016/S1359-6446(00)01511-7 contributor: fullname: Harvey – volume: 6 start-page: 255 year: 2001 ident: 10.1177/1087057103259346_bib37 article-title: A cGMP-dependent protein kinase assay for high throughput screening based on time-resolved fluorescence resonance energy transfer publication-title: J Biomol Screen doi: 10.1177/108705710100600407 contributor: fullname: Bader – volume: 5 start-page: 23 year: 2000 ident: 10.1177/1087057103259346_bib27 article-title: Identification of a high-affinity anti-phosphoserine antibody for the development of a homogeneous fluorescence polarization assay of protein kinase c publication-title: J Biomol Screen doi: 10.1177/108705710000500106 contributor: fullname: Wu – volume: 8 start-page: 176 year: 2003 ident: 10.1177/1087057103259346_bib31 article-title: Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 kinases, 1 protease, and 1 phosphatase publication-title: J Biomol Screen doi: 10.1177/1087057103252304 contributor: fullname: Turek-Etienne – volume: 1526 start-page: 257 year: 2001 ident: 10.1177/1087057103259346_bib38 article-title: Expression, purification, characterization and homology modeling of active AKT/PKB, a key enzyme involved in cell survival signaling publication-title: Biochim Biophys Acta doi: 10.1016/S0304-4165(01)00143-X contributor: fullname: Kumar – volume: 3603 start-page: 251 year: 1999 ident: 10.1177/1087057103259346_bib26 article-title: A robust, versatile tyrosine kinase assay for HTS in drug discovery publication-title: Prog Biomed Optics (SPIE) contributor: fullname: Deshpande – volume: 269 start-page: 94 year: 1999 ident: 10.1177/1087057103259346_bib33 article-title: Homogeneous proximity tyrosine kinase assays: scintillation proximity assay versus homogeneous time-resolved fluorescence publication-title: Anal Biochem doi: 10.1006/abio.1999.4029 contributor: fullname: Park – volume: 268 start-page: 318 year: 1999 ident: 10.1177/1087057103259346_bib32 article-title: A scintillation proximity assay for the Raf/MEK/ERK kinase cascade: high-throughput screening and identification of selective enzyme inhibitors publication-title: Anal Biochem doi: 10.1006/abio.1998.3030 contributor: fullname: McDonald – volume: 56 start-page: 428 year: 2002 ident: 10.1177/1087057103259346_bib5 article-title: Glivec: a new treatment modality for CML: a case history publication-title: Chimia contributor: fullname: Zimmermann – volume: 411 start-page: 355 year: 2001 ident: 10.1177/1087057103259346_bib8 article-title: Oncogenic kinase signalling publication-title: Nature doi: 10.1038/35077225 contributor: fullname: Blume-Jensen – volume: 274 start-page: 21528 year: 1999 ident: 10.1177/1087057103259346_bib15 article-title: Up-regulation of AKT3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines publication-title: J Biol Chem doi: 10.1074/jbc.274.31.21528 contributor: fullname: Nakatani – volume: 111 start-page: 293 year: 2002 ident: 10.1177/1087057103259346_bib6 article-title: PKB binding proteins: getting in on the AKT publication-title: Cell doi: 10.1016/S0092-8674(02)01083-8 contributor: fullname: Brazil – volume: 255 start-page: 257 year: 1998 ident: 10.1177/1087057103259346_bib25 article-title: A fluorescence polarization competition immunoassay for tyrosine kinases publication-title: Anal Biochem doi: 10.1006/abio.1997.2455 contributor: fullname: Seethala – volume: 91 start-page: 231 year: 1997 ident: 10.1177/1087057103259346_bib10 article-title: AKT phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery publication-title: Cell doi: 10.1016/S0092-8674(00)80405-5 contributor: fullname: Datta – volume: 7 start-page: 191 year: 2002 ident: 10.1177/1087057103259346_bib30 article-title: Comparison of assay technologies for a tyrosine kinase assay generates different results in high-throughput screening publication-title: J Biomol Screen doi: 10.1177/108705710200700304 contributor: fullname: Sills – volume: 5 start-page: 713 year: 2002 ident: 10.1177/1087057103259346_bib2 article-title: Kinases as targets: prospects for chronic therapy publication-title: Curr Opin Drug Discov Dev contributor: fullname: Orchard – volume: 2 start-page: 143 year: 2002 ident: 10.1177/1087057103259346_bib18 article-title: Natural products in cancer chemotherapy: past, present and future publication-title: Nat Rev Cancer doi: 10.1038/nrc723 contributor: fullname: Mann – volume: 38 start-page: 643 year: 1999 ident: 10.1177/1087057103259346_bib19 article-title: Statistical investigation into the structure complementarity of natural products and synthetic compounds publication-title: Angew Chem Int Ed doi: 10.1002/(SICI)1521-3773(19990301)38:5<643::AID-ANIE643>3.0.CO;2-G contributor: fullname: Henkel – volume: 5 start-page: 463 year: 2000 ident: 10.1177/1087057103259346_bib34 article-title: Development of a homogeneous time-resolved fluorescence assay for high throughput screening to identify Lck inhibitors: comparison with scintillation proximity assay and streptavidin-coated plate assay publication-title: J Biomol Screen doi: 10.1177/108705710000500609 contributor: fullname: Ohmi |
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| SubjectTerms | Biological Factors - metabolism Chromatography, High Pressure Liquid Fermentation Fluorescence Polarization - methods Fluorescent Dyes - chemistry Protein-Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins - metabolism Proto-Oncogene Proteins c-akt Spectrophotometry, Ultraviolet |
| Title | Use of red-shifted dyes in a fluorescence polarization AKT kinase assay for detection of biological activity in natural product extracts |
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