Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing application

• Published in: Chemical science (Cambridge) Vol. 12; no. 47; pp. 15750 - 15756
• Main Authors: Chen, Zhijie, Wang, Zhenqin, Xu, Yang, Zhang, Xiaochun, Tian, Boxue, Bai, Jingwei
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 08.12.2021; The Royal Society of Chemistry
• Subjects: Amino acids; Chemistry; Commercialization; Nucleic acids; Peptides; Phosphorylation; Proteins

The lack of an efficient, low-cost sequencing method has long been a significant bottleneck in protein research and applications. In recent years, the nanopore platform has emerged as a fast and inexpensive method for single-molecule nucleic acid sequencing, but attempts to apply it to protein/pepti...