Hypertonicity Induces Injury to Cultured Human Endothelium: Attenuation by Glutamine

• Published in: The Annals of thoracic surgery Vol. 64; no. 6; pp. 1770 - 1775
• Main Authors: Parolari, Alessandro, Sala, Roberto, Antona, Carlo, Bussolati, Ovidio, Alamanni, Francesco, Mezzadri, Paola, Dall’Asta, Valeria, Gazzola, Gian C, Biglioli, Paolo
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.1997; Elsevier Science
• Subjects: Aged; Apoptosis; Biological and medical sciences; Cardiovascular system; Cell Survival - drug effects; Cells, Cultured; confocal microscopy; Culture Media; endothelial cells; Endothelium, Vascular - drug effects; Endothelium, Vascular - pathology; glutamine; Glutamine - pharmacology; Humans; Hypertonic Solutions - pharmacology; hypertonicity; In Vitro Techniques; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Microscopy, Confocal; Middle Aged; Osmotic Pressure; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Saphenous Vein - drug effects; endothelial cells; glutamine; confocal microscopy; hypertonicity; Human; Culture medium; Cell culture; Endothelial cell; Exploration; Concentration; Endothelium; Relation; Saphenous vein; Blood vessel; Circulatory system; Hypertonic solution; Viability; Glutamine

Background. Although most preservation solutions as well as some cardioplegic solutions used for organ storage and transplantation are hypertonic, the effects of extracellular hypertonicity on endothelium are not well established. Aims of this study were to evaluate the response of cultured human sa...