Competitive fluorescent immunosensor based on catalytic hairpin self-assembly for multiresidue detection of organophosphate pesticides in agricultural products

• Published in: Food chemistry Vol. 413; p. 135607
• Main Authors: Wang, Yuanshang, Abd El-Aty, A.M., Wang, Shanshan, Cui, Xueyan, Zhao, Jing, Lei, Xingmei, Xu, Lingyuan, She, Yongxin, Jin, Fen, Eun, Jong-Bang, Shim, Jae-Han, Wang, Jing, Jin, Maojun, Hammock, Bruce D.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2023
• Subjects: Biosensing Techniques; Catalyzed hairpin self-assembly; Chlorpyrifos; Chromatography, Liquid - methods; Fluorescent immunosensors; Gold nanoparticles; Immunoassay; Insecticides - analysis; Multiresidue detection; Organophosphate pesticides; Pesticides - analysis; Tandem Mass Spectrometry; Fluorescent immunosensors; Catalyzed hairpin self-assembly; Organophosphate pesticides; Multiresidue detection; Gold nanoparticles

•Dual signal amplification was achieved by combining immunoassay and nucleic acid amplification.•The method could occur at a constant temperature without enzyme catalysis.•The detection limit was as low as 0.012, 0.0057, and 0.0074 ng/mL.•Signals were detected through different fluorescent groups mo...