Retention of enzyme by electropolymerized film: a new approach in developing a hypoxanthine biosensor

An amperometric biosensor for hypoxanthine was constructed by forming a layer of crosslinked xanthine oxidase on a platinum electrode, followed by electropolymerization of a submonolayer film of resorcinol and para-diaminobenzene. The fabricated electrodes were evaluated for speed of response, sensi...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology and bioengineering Vol. 37; no. 8; p. 729
Main Authors Nguyen, A L, Luong, J H, Yacynych, A M
Format Journal Article
LanguageEnglish
Published United States 05.04.1991
Online AccessGet more information

Cover

Abstract An amperometric biosensor for hypoxanthine was constructed by forming a layer of crosslinked xanthine oxidase on a platinum electrode, followed by electropolymerization of a submonolayer film of resorcinol and para-diaminobenzene. The fabricated electrodes were evaluated for speed of response, sensitivity, and reusability. Optimal performance was obtained with enzyme-based electrodes sparsely covered with film which was formed by electropolymerization in less than 6 min. The resulting electrodes exhibited linear response to hypoxanthine in the. range 5-300 microM with a response time of 2 min. Application of the biosensor in monitoring hypoxanthine content of fish extracts yielded results which agreed well with spectrophotometric assays using soluble xanthine oxidase. The biosensor was stable for 60 days when stored at 4 degrees C in phosphate buffer and it could be used continuously for 6 h with over 50 assays.
AbstractList An amperometric biosensor for hypoxanthine was constructed by forming a layer of crosslinked xanthine oxidase on a platinum electrode, followed by electropolymerization of a submonolayer film of resorcinol and para-diaminobenzene. The fabricated electrodes were evaluated for speed of response, sensitivity, and reusability. Optimal performance was obtained with enzyme-based electrodes sparsely covered with film which was formed by electropolymerization in less than 6 min. The resulting electrodes exhibited linear response to hypoxanthine in the. range 5-300 microM with a response time of 2 min. Application of the biosensor in monitoring hypoxanthine content of fish extracts yielded results which agreed well with spectrophotometric assays using soluble xanthine oxidase. The biosensor was stable for 60 days when stored at 4 degrees C in phosphate buffer and it could be used continuously for 6 h with over 50 assays.
Author Luong, J H
Nguyen, A L
Yacynych, A M
Author_xml – sequence: 1
  givenname: A L
  surname: Nguyen
  fullname: Nguyen, A L
  organization: Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Canada
– sequence: 2
  givenname: J H
  surname: Luong
  fullname: Luong, J H
– sequence: 3
  givenname: A M
  surname: Yacynych
  fullname: Yacynych, A M
BackLink https://www.ncbi.nlm.nih.gov/pubmed/18600669$$D View this record in MEDLINE/PubMed
BookMark eNo1j0tLxDAUhbMYcR66dCv5Ax1v07zqTgZfMCCIroc0vbWRNglpfXR-vQV1dTjngw_Omix88EjIRQ7bHIBdVW7cMgmFAg1qQVYAILNClGxJ1sPwPlelpTwly1zLGclyRfAZR_SjC56GhqI_Tj3SaqLYoR1TiKGbh-SOWNPGdf01NdTjFzUxpmBsS52nNX5iF6LzbzNspxi-jR9b52ePCwP6IaQzctKYbsDzv9yQ17vbl91Dtn-6f9zd7DPLFVOZBVQGc8sl8AJRaw7CKlUXjay5Zaxk3HKrrDasKEVVcSVMrrU1BqXgjWAbcvnrjR9Vj_UhJtebNB3-_7IfMnNZFQ
CitedBy_id crossref_primary_10_1002__SICI_1521_4109_199804_10_4_273__AID_ELAN273_3_0_CO_2_O
crossref_primary_10_1016_S0956_5663_98_00029_3
crossref_primary_10_1002_elan_1140071002
crossref_primary_10_1016_S0925_4005_98_00285_8
crossref_primary_10_1021_cr010423z
crossref_primary_10_1016_j_snb_2005_03_001
crossref_primary_10_1111_j_1365_2621_1992_tb05429_x
crossref_primary_10_1016_0308_8146_95_93297_5
crossref_primary_10_1016_0956_5663_93_80027_M
crossref_primary_10_1016_j_foodchem_2010_04_066
crossref_primary_10_1016_0956_5663_94_80033_2
crossref_primary_10_1016_0003_2670_93_80221_6
crossref_primary_10_1016_0141_0229_92_90169_O
crossref_primary_10_1016_0956_5663_94_80025_1
crossref_primary_10_1016_S0003_2670_00_00739_X
crossref_primary_10_1016_0956_5663_94_80055_3
crossref_primary_10_1111_j_1745_4514_2005_00050_x
crossref_primary_10_1002_elan_1140050506
crossref_primary_10_1007_BF02916436
ContentType Journal Article
DBID NPM
DOI 10.1002/bit.260370807
DatabaseName PubMed
DatabaseTitle PubMed
DatabaseTitleList PubMed
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Engineering
Chemistry
Biology
Anatomy & Physiology
ExternalDocumentID 18600669
Genre Journal Article
GroupedDBID ---
-~X
.GJ
0R~
1L6
1OB
1OC
1ZS
23N
33P
3EH
3SF
3WU
4ZD
50Y
50Z
51W
51X
52M
52N
52O
52P
52T
52U
52W
52X
53G
5GY
5RE
5VS
66C
702
7PT
8-0
8-1
8UM
AAESR
ABCQN
ABEML
ABJNI
ACBWZ
ACGFO
ACGFS
ACIWK
ACPRK
ACSCC
ACXQS
ADKYN
ADOZA
ADZMN
AENEX
AEUQT
AFBPY
AFFNX
AFRAH
AI.
AIAGR
ALAGY
ALMA_UNASSIGNED_HOLDINGS
AMBMR
ASPBG
ATUGU
AVWKF
AZFZN
BAFTC
BDRZF
BFHJK
BLYAC
BNHUX
BY8
CS3
D-E
D-F
DCZOG
DR1
DR2
DU5
EBS
EJD
F04
F5P
FEDTE
GNP
GODZA
H.T
H.X
HBH
HHY
HHZ
HVGLF
HZ~
IX1
J0M
JPC
KQQ
LATKE
LAW
LC2
LC3
LEEKS
LH6
LOXES
LUTES
LW6
MK4
MRFUL
N04
N05
N9A
NDZJH
NF~
NNB
NPM
O66
O9-
OIG
P2P
P2W
P2X
PALCI
PQQKQ
Q.N
QRW
RBB
RIWAO
RJQFR
RWI
RX1
SAMSI
SUPJJ
TN5
VH1
W8V
W99
WBKPD
WH7
WIB
WIH
WJL
WNSPC
WSB
WXSBR
XPP
XV2
Y6R
ZXP
~02
~KM
~WT
ID FETCH-LOGICAL-c4727-c0e7ae1c46043ee88405c77d3f6d4c22924c4c7c8a2395bb475a188caae654f52
ISSN 0006-3592
IngestDate Sat Sep 28 07:47:11 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 8
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c4727-c0e7ae1c46043ee88405c77d3f6d4c22924c4c7c8a2395bb475a188caae654f52
PMID 18600669
ParticipantIDs pubmed_primary_18600669
PublicationCentury 1900
PublicationDate 5 April 1991
PublicationDateYYYYMMDD 1991-04-05
PublicationDate_xml – month: 04
  year: 1991
  text: 5 April 1991
  day: 05
PublicationDecade 1990
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Biotechnology and bioengineering
PublicationTitleAlternate Biotechnol Bioeng
PublicationYear 1991
SSID ssj0007866
Score 1.5217742
Snippet An amperometric biosensor for hypoxanthine was constructed by forming a layer of crosslinked xanthine oxidase on a platinum electrode, followed by...
SourceID pubmed
SourceType Index Database
StartPage 729
Title Retention of enzyme by electropolymerized film: a new approach in developing a hypoxanthine biosensor
URI https://www.ncbi.nlm.nih.gov/pubmed/18600669
Volume 37
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwELW2VAh6QLClpRSQD4hLlbLZ-Cu9LRWoQqgH1ErlVNmO3UbqJhFkJbK_nnHsZLMtCGgOURRHluN5mbwZz4wResusSRWjJMoUYxEBoyOSKUmizGpqNTGa6DZA9pSdnJPPF_RiNKoGUUuLWh3q5W_zSu4jVbgHcnVZsv8h2b5TuAHXIF84g4Th_E8y_uoYb0f5TLFs5sbRybC1TVXeNG49Zgmc0uY3c5_XDDS6LyTuQ2H7rCl5cN1U5U-Y62vHPVVe_gAjt_y-tvCbl3XvjW9XHuAxsypq2DuXrxaN12izg96__GXRBQCvciK-Sd0Ujd-QahZ8s1lIy4vb4BW6pllZlNB0TbP6ci4BQWKgJrn3ctxR374crMrrQzCzEg5slg-fg3mq5q0sY8EcWUr_3nqrmnbXtIE2uHB68dR5d8Kfmwu_tt29S6jJCqN6vzYmV2k29HPLGmlZydlT9CSYE3jmsfEMjUwxRtuzQtblvMHvcBvg28pqjB5-6K4eHXfb_I3R1qAi5TYyPaRwabGHFFYNvgsp7CB1hCUGQOEOUDgv8ApQ0DgEFO4B9Rydf_p4dnwShZ04Ik2A4EZ6Yrg0sSZsQhJjhACarznPEssyoqdTMOLho-ZayGmSUqUIpzIWQktpQA9YOt1BD4qyMC8QJhRMdCrhYIaoOFUZn1joPI0N13HM99Cun9DLypdbueym-uUfW_bR4xUmX6FNC9-3eQ1ksVZvWgn_ArLWaxs
link.rule.ids 783
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Retention+of+enzyme+by+electropolymerized+film%3A+a+new+approach+in+developing+a+hypoxanthine+biosensor&rft.jtitle=Biotechnology+and+bioengineering&rft.au=Nguyen%2C+A+L&rft.au=Luong%2C+J+H&rft.au=Yacynych%2C+A+M&rft.date=1991-04-05&rft.issn=0006-3592&rft.volume=37&rft.issue=8&rft.spage=729&rft_id=info:doi/10.1002%2Fbit.260370807&rft_id=info%3Apmid%2F18600669&rft_id=info%3Apmid%2F18600669&rft.externalDocID=18600669
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3592&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3592&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3592&client=summon