Salt‐enhanced cultivation as a morphology engineering tool for filamentous actinomycetes: Increased production of labyrinthopeptin A1 in Actinomadura namibiensis
Salt‐enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500‐mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the...
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| Published in | Engineering in life sciences Vol. 19; no. 11; pp. 781 - 794 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Germany
John Wiley and Sons Inc
01.11.2019
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1618-0240 1618-2863 |
| DOI | 10.1002/elsc.201900036 |
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| Abstract | Salt‐enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500‐mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH4)2SO4 led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH4)2SO4, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non‐supplemented control, resulting in 325 mg L−1 labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium‐ and sulfate‐containing salts (e.g., NH4Cl, K2SO4) was much lower than the performance of (NH4)2SO4. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth‐associated labyrinthopeptin productivity was found. The change in the cell morphology of A. namibiensis in conjunction with increased osmolality by the addition of 50 mM (NH4)2SO4, was quantified by image analysis. A. namibiensis always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non‐supplemented control, the morphology of (NH4)2SO4‐supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. |
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| AbstractList | Salt‐enhanced cultivation as a morphology engineering tool for the filamentous actinomycete
Actinomadura namibiensis
was evaluated in 500‐mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH
4
)
2
SO
4
led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH
4
)
2
SO
4
, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non‐supplemented control, resulting in 325 mg L
−1
labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium‐ and sulfate‐containing salts (e.g., NH
4
Cl, K
2
SO
4
) was much lower than the performance of (NH
4
)
2
SO
4
. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth‐associated labyrinthopeptin productivity was found. The change in the cell morphology of
A. namibiensis
in conjunction with increased osmolality by the addition of 50 mM (NH
4
)
2
SO
4
, was quantified by image analysis.
A. namibiensis
always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non‐supplemented control, the morphology of (NH
4
)
2
SO
4
‐supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. Salt-enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500-mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH4)2SO4 led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH4)2SO4, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non-supplemented control, resulting in 325 mg L-1 labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium- and sulfate-containing salts (e.g., NH4Cl, K2SO4) was much lower than the performance of (NH4)2SO4. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth-associated labyrinthopeptin productivity was found. The change in the cell morphology of A. namibiensis in conjunction with increased osmolality by the addition of 50 mM (NH4)2SO4, was quantified by image analysis. A. namibiensis always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non-supplemented control, the morphology of (NH4)2SO4-supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase.Salt-enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500-mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH4)2SO4 led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH4)2SO4, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non-supplemented control, resulting in 325 mg L-1 labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium- and sulfate-containing salts (e.g., NH4Cl, K2SO4) was much lower than the performance of (NH4)2SO4. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth-associated labyrinthopeptin productivity was found. The change in the cell morphology of A. namibiensis in conjunction with increased osmolality by the addition of 50 mM (NH4)2SO4, was quantified by image analysis. A. namibiensis always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non-supplemented control, the morphology of (NH4)2SO4-supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. Salt-enhanced cultivation as a morphology engineering tool for the filamentous actinomycete was evaluated in 500-mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH ) SO led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH ) SO , the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non-supplemented control, resulting in 325 mg L labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium- and sulfate-containing salts (e.g., NH Cl, K SO ) was much lower than the performance of (NH ) SO . A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth-associated labyrinthopeptin productivity was found. The change in the cell morphology of in conjunction with increased osmolality by the addition of 50 mM (NH ) SO , was quantified by image analysis. always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non-supplemented control, the morphology of (NH ) SO -supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. Salt‐enhanced cultivation as a morphology engineering tool for the filamentous actinomycete Actinomadura namibiensis was evaluated in 500‐mL shaking flasks (working volume 100 mL) with the aim of increasing the concentration of the pharmaceutically interesting peptide labyrinthopeptin A1. Among the inorganic salts added to a complex production medium, the addition of (NH4)2SO4 led to the highest amount of labyrinthopeptin A1 production. By using 50 mM (NH4)2SO4, the labyrinthopeptin A1 concentration increased up to sevenfold compared to the non‐supplemented control, resulting in 325 mg L−1 labyrinthopeptin A1 after 10 days of cultivation. The performance of other ammonium‐ and sulfate‐containing salts (e.g., NH4Cl, K2SO4) was much lower than the performance of (NH4)2SO4. A positive correlation between the uptake of glycerol as one of the main carbon sources and nongrowth‐associated labyrinthopeptin productivity was found. The change in the cell morphology of A. namibiensis in conjunction with increased osmolality by the addition of 50 mM (NH4)2SO4, was quantified by image analysis. A. namibiensis always developed a heterogeneous morphology with pellets and loose mycelia present simultaneously. In contrast to the non‐supplemented control, the morphology of (NH4)2SO4‐supplemented cultures was characterized by smaller and circular pellets that were more stable against disintegration in the stationary production phase. |
| Author | Büchs, Jochen Krull, Rainer Tesche, Sebastian Rösemeier‐Scheumann, René Lohr, Jonas Hanke, René |
| AuthorAffiliation | 2 Center of Pharmaceutical Engineering (PVZ) Technische Universität Braunschweig Braunschweig Germany 1 Institute of Biochemical Engineering Technische Universität Braunschweig Braunschweig Germany 3 AVT ‐ Chair of Biochemical Engineering RWTH Aachen University Aachen Germany |
| AuthorAffiliation_xml | – name: 1 Institute of Biochemical Engineering Technische Universität Braunschweig Braunschweig Germany – name: 2 Center of Pharmaceutical Engineering (PVZ) Technische Universität Braunschweig Braunschweig Germany – name: 3 AVT ‐ Chair of Biochemical Engineering RWTH Aachen University Aachen Germany |
| Author_xml | – sequence: 1 givenname: Sebastian surname: Tesche fullname: Tesche, Sebastian organization: Technische Universität Braunschweig – sequence: 2 givenname: René surname: Rösemeier‐Scheumann fullname: Rösemeier‐Scheumann, René organization: Technische Universität Braunschweig – sequence: 3 givenname: Jonas surname: Lohr fullname: Lohr, Jonas organization: Technische Universität Braunschweig – sequence: 4 givenname: René surname: Hanke fullname: Hanke, René organization: RWTH Aachen University – sequence: 5 givenname: Jochen surname: Büchs fullname: Büchs, Jochen organization: RWTH Aachen University – sequence: 6 givenname: Rainer surname: Krull fullname: Krull, Rainer email: r.krull@tu-braunschweig.de organization: Technische Universität Braunschweig |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32624971$$D View this record in MEDLINE/PubMed |
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| Keywords | actinomycete morphology engineering labyrinthopeptin salt‐enhanced cultivation image analysis ammonium sulfate |
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| SubjectTerms | actinomycete ammonium sulfate image analysis labyrinthopeptin morphology engineering salt‐enhanced cultivation |
| Title | Salt‐enhanced cultivation as a morphology engineering tool for filamentous actinomycetes: Increased production of labyrinthopeptin A1 in Actinomadura namibiensis |
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