LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochrome P450s

• Published in: Chembiochem : a European journal of chemical biology Vol. 11; no. 7; pp. 987 - 994
• Main Authors: Sabbadin, Federico, Hyde, Ralph, Robin, Aelig, Hilgarth, Eva-Maria, Delenne, Marie, Flitsch, Sabine, Turner, Nicholas, Grogan, Gideon, Bruce, Neil C
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 03.05.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: biocatalysis; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; cytochromes; Electron Transport; enzyme catalysis; Enzymes; Genetic Vectors; Heme - chemistry; heme proteins; high throughput; Kinetics; NADP - chemistry; Oxidation-Reduction; Protein Structure, Tertiary; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism

Cytochromes P450 (P450s) are a family of haem-containing oxidases with considerable potential as tools for industrial biocatalysis. Organismal genomes are revealing thousands of gene sequences that encode P450s of as yet unknown function, the exploitation of which will require high-throughput tools...