A Parallel Reaction Monitoring Mass Spectrometric Method for Analysis of Potential CSF Biomarkers for Alzheimer's Disease

• Published in: Proteomics. Clinical applications Vol. 12; no. 1
• Main Authors: Brinkmalm, Gunnar, Sjödin, Simon, Simonsen, Anja Hviid, Hasselbalch, Steen Gregers, Zetterberg, Henrik, Brinkmalm, Ann, Blennow, Kaj
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.01.2018
• Subjects: Aged; Alzheimer Disease - cerebrospinal fluid; Alzheimer Disease - diagnosis; Alzheimer Disease - genetics; Alzheimer Disease - pathology; Alzheimer's disease; Amino Acid Sequence; Amyloid precursor protein; biomarker; Biomarkers; Biomarkers - cerebrospinal fluid; C-Reactive Protein - cerebrospinal fluid; C-Reactive Protein - genetics; Case-Control Studies; Cell Adhesion Molecules - cerebrospinal fluid; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules, Neuronal - cerebrospinal fluid; Cell Adhesion Molecules, Neuronal - genetics; Cerebrospinal fluid; Chromatography, Liquid; Chromogranin A - cerebrospinal fluid; Chromogranin A - genetics; Coefficient of variation; Core protein; Cystatin C; Female; Gene Expression; Humans; Immune system; Innate immunity; Lysozyme; Male; Mass spectrometry; Mass spectroscopy; Middle Aged; Monitoring; Nerve Growth Factors - cerebrospinal fluid; Nerve Growth Factors - genetics; Nerve Tissue Proteins - cerebrospinal fluid; Nerve Tissue Proteins - genetics; Neurocan; Neurodegenerative diseases; Neurological diseases; Neurosciences; Neurovetenskaper; parallel reaction monitoring; Pentraxins; Peptide Fragments - cerebrospinal fluid; Peptides; Pilot Projects; Proteins; Proteolysis; Proteomics - methods; Secretogranin II - cerebrospinal fluid; Secretogranin II - genetics; Tandem Mass Spectrometry - methods; mass spectrometry; biomarker; Alzheimer's disease; parallel reaction monitoring; cerebrospinal fluid
• ISSN: 1862-8346; 1862-8354; 1862-8354
• DOI: 10.1002/prca.201700131

Scope The aim of this study was to develop and evaluate a parallel reaction monitoring mass spectrometry (PRM‐MS) assay consisting of a panel of potential protein biomarkers in cerebrospinal fluid (CSF). Experimental design Thirteen proteins were selected based on their association with neurodegener...