Sites of reactive oxygen species generation by mitochondria oxidizing different substrates

• Published in: Redox biology Vol. 1; no. 1; pp. 304 - 312
• Main Authors: Quinlan, Casey L., Perevoshchikova, Irina V., Hey-Mogensen, Martin, Orr, Adam L., Brand, Martin D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier 01.01.2013
• Subjects: Animals; Cytochrome b; Electron Transport Complex I - chemistry; Electron Transport Complex I - metabolism; Electron Transport Complex III - chemistry; Electron Transport Complex III - metabolism; Female; Glycerophosphates - metabolism; Hydrogen peroxide; Malates - metabolism; Mitochondria, Muscle - metabolism; Muscle, Skeletal - metabolism; NADH; Palmitoylcarnitine - metabolism; Rats; Rats, Wistar; Reactive Oxygen Species - metabolism; Research Paper; Respiratory complexes; Succinic Acid - metabolism; Superoxide; Superoxides - metabolism; Ubiquinone; IF, flavin site of complex I; QH2, ubiquinol; OGDH, 2-oxoglutarate dehydrogenase; Hydrogen peroxide; Respiratory complexes; IIIQo, quinol oxidation site of complex III; Superoxide; Eh, redox potential; IQ, quinone-binding site of complex I; Q, ubiquinone; Cytochrome b; ETF, electron transferring flavoprotein; NADH; Ubiquinone; ETF:QOR, ETF:ubiquinone oxidoreductase; IIF, flavin site of complex II; PDH, pyruvate dehydrogenase; mGPDH, mitochondrial glycerol 3-phosphate dehydrogenase; ROS, reactive oxygen species; CDNB, 1-chloro-2,4-dinitrobenzene

Mitochondrial radical production is important in redox signaling, aging and disease, but the relative contributions of different production sites are poorly understood. We analyzed the rates of superoxide/H2O2 production from different defined sites in rat skeletal muscle mitochondria oxidizing a va...