Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

•Antibiotic resistance genes in the raw and treated sewage were demonstrated.•mecA gene was present in both raw and treated sewage in all sampling events.•Genetic transformation was demonstrated using the mecA gene found in sewage. Increasing uses and disposals of antibiotics to the environment have...

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Published inBioresource technology Vol. 188; pp. 79 - 83
Main Authors Naquin, Anthony, Shrestha, Arsen, Sherpa, Mingma, Nathaniel, Rajkumar, Boopathy, Raj
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.07.2015
Subjects
Anti-Bacterial Agents - chemistry
antibiotic resistance
Antibiotic resistance genes
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological Oxygen Demand Analysis
Carbon - chemistry
DNA, Bacterial - chemistry
Drug Resistance, Bacterial - genetics
Erythromycin
Erythromycin - chemistry
genes
genetic transformation
Hydrogen-Ion Concentration
Louisiana
methicillin
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Microbial Sensitivity Tests
MRSA
Penicillin-Binding Proteins
Refuse Disposal - methods
RNA, Ribosomal, 16S - genetics
sewage
Sewage - microbiology
Sewage treatment
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Tetracycline
Tetracycline - chemistry
Waste Water - microbiology
wastewater treatment
Water Microbiology
Louisiana
Erythromycin
Antibiotic resistance genes
MRSA
Sewage treatment
Tetracycline
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Abstract •Antibiotic resistance genes in the raw and treated sewage were demonstrated.•mecA gene was present in both raw and treated sewage in all sampling events.•Genetic transformation was demonstrated using the mecA gene found in sewage. Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater treatment plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town sewage treatment plant. Therefore, this study was conducted using raw sewage as well as treated sewage from a sewage treatment plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux sewage treatment plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.
AbstractList •Antibiotic resistance genes in the raw and treated sewage were demonstrated.•mecA gene was present in both raw and treated sewage in all sampling events.•Genetic transformation was demonstrated using the mecA gene found in sewage. Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater treatment plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town sewage treatment plant. Therefore, this study was conducted using raw sewage as well as treated sewage from a sewage treatment plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux sewage treatment plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.
Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater treatment plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town sewage treatment plant. Therefore, this study was conducted using raw sewage as well as treated sewage from a sewage treatment plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux sewage treatment plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater treatment plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town sewage treatment plant. Therefore, this study was conducted using raw sewage as well as treated sewage from a sewage treatment plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux sewage treatment plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.
Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of antibiotic resistance is wastewater treatment plant, where bacteria and antibiotics can come in contact and can acquire antibiotics resistance. There are very few studies on this subject from a small town sewage treatment plant. Therefore, this study was conducted using raw sewage as well as treated sewage from a sewage treatment plant in Thibodaux in rural southeast Louisiana in USA. Samples were collected monthly from the Thibodaux sewage treatment plant and the presence of antibiotic resistance genes was monitored. The study showed the presence of antibiotic resistance genes in both raw and treated sewage in every month of the study period. The genetic transformation assay showed the successful transformation of methicillin resistant gene, mecA to an antibiotic sensitive Staphylococcus aureus, which became antibiotic resistant within 24h.
Author Shrestha, Arsen
Nathaniel, Rajkumar
Sherpa, Mingma
Boopathy, Raj
Naquin, Anthony
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  surname: Boopathy
  fullname: Boopathy, Raj
  email: Ramaraj.Boopathy@nicholls.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25662190$$D View this record in MEDLINE/PubMed
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Cites_doi 10.2166/wst.2012.204
10.1080/15287390600975137
10.1016/j.watres.2006.11.045
10.1128/AAC.43.6.1523
10.1016/j.watres.2010.08.033
10.3389/fmicb.2013.00017
10.1007/s00253-010-2583-6
10.1128/AAC.36.2.429
10.1021/es803309m
10.1021/es301145m
10.1016/j.ecoenv.2013.01.016
10.1021/es102765a
10.1128/AAC.43.12.2823
10.1128/AEM.02898-09
10.1016/j.scitotenv.2013.01.032
10.1086/533591
10.1016/j.chemosphere.2011.09.048
10.1016/j.ibiod.2014.05.028
10.1021/es202775r
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Keywords Erythromycin
Antibiotic resistance genes
MRSA
Sewage treatment
Tetracycline
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References Novo, Manaia (b0095) 2010; 87
Everage, Boopathy, Nathaniel, LaFleur, Doucet (b0045) 2014; 95
Diel, LaPara (b0035) 2010; 44
Center for Disease Control (CDC), 2014. Antibiotic resistance threats in the United States. <http//:CDC.gov> (accessed 27.10.14).
Zhang, Zhang, Zhang, Fang (b0130) 2009; 43
Kim, Aga (b0060) 2007; 10
Liu, Zhang, Yang, Tian, Ren, Zhang (b0075) 2012; 46
Thevenon, Adatte, Wildi, Poté (b0125) 2012; 86
(accessed 11.11.14).
Brown (b0020) 2005
Roberts, Sutcliffe, Courvalin, Jensen, Rood, Seppala (b0110) 1999; 43
Auerbach, Seyfried, McMahoon (b0010) 2007; 41
Spellberg, Blaser, Guidos, Boucher, Bradley, Eiesnstein (b0115) 2011; 525
Louisiana Department of Wildlife and Fisheries, 2013. Statistics of commercial license sales from 1987–2011.
Suzuki, Hiramatsu, Yokota (b0120) 1992; 36
LaPara, Burch, McNamara, Tan, Yan, Eichmiller (b0065) 2011; 45
Galvin, Boyle, Hickey, Vellinga, Morris, Cormican (b0050) 2010; 76
Dzidic, Bedekovic (b0040) 2003; 6
Morell, Balkin (b0085) 2010; 83
APHA (b0005) 1998
Rizzo, Manaia, Merlin, Schwartz, Dagot, Ploy, Michael, Fatta-Kassions (b0105) 2013; 447
Bouki, Venieri, Diamadopoulos (b0015) 2013; 91
Burch, Sadowski, LaPara (b0025) 2013; 4
Gordon, Lowy (b0055) 2008; 46
Munir, Wong, Xagoraraki (b0090) 2011; 45
Levy, McMurry, Barbosa, Burdett, Courvalin, Hillen (b0070) 1999; 43
Pozo, Villamar, Martínez, Vidal (b0100) 2012; 66
Levy (10.1016/j.biortech.2015.01.052_b0070) 1999; 43
Brown (10.1016/j.biortech.2015.01.052_b0020) 2005
Bouki (10.1016/j.biortech.2015.01.052_b0015) 2013; 91
Thevenon (10.1016/j.biortech.2015.01.052_b0125) 2012; 86
Burch (10.1016/j.biortech.2015.01.052_b0025) 2013; 4
10.1016/j.biortech.2015.01.052_b0030
Gordon (10.1016/j.biortech.2015.01.052_b0055) 2008; 46
Roberts (10.1016/j.biortech.2015.01.052_b0110) 1999; 43
Novo (10.1016/j.biortech.2015.01.052_b0095) 2010; 87
Rizzo (10.1016/j.biortech.2015.01.052_b0105) 2013; 447
Everage (10.1016/j.biortech.2015.01.052_b0045) 2014; 95
Morell (10.1016/j.biortech.2015.01.052_b0085) 2010; 83
Spellberg (10.1016/j.biortech.2015.01.052_b0115) 2011; 525
Suzuki (10.1016/j.biortech.2015.01.052_b0120) 1992; 36
Diel (10.1016/j.biortech.2015.01.052_b0035) 2010; 44
Dzidic (10.1016/j.biortech.2015.01.052_b0040) 2003; 6
Galvin (10.1016/j.biortech.2015.01.052_b0050) 2010; 76
Liu (10.1016/j.biortech.2015.01.052_b0075) 2012; 46
Zhang (10.1016/j.biortech.2015.01.052_b0130) 2009; 43
Kim (10.1016/j.biortech.2015.01.052_b0060) 2007; 10
LaPara (10.1016/j.biortech.2015.01.052_b0065) 2011; 45
10.1016/j.biortech.2015.01.052_b0080
Munir (10.1016/j.biortech.2015.01.052_b0090) 2011; 45
Pozo (10.1016/j.biortech.2015.01.052_b0100) 2012; 66
Auerbach (10.1016/j.biortech.2015.01.052_b0010) 2007; 41
APHA (10.1016/j.biortech.2015.01.052_b0005) 1998
References_xml – volume: 45
  start-page: 681
  year: 2011
  end-page: 693
  ident: b0090
  article-title: Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan
  publication-title: Water Res.
– volume: 44
  start-page: 9128
  year: 2010
  end-page: 9133
  ident: b0035
  article-title: Effect of temperature on the fate of genes encoding tetracycline resistance and the integrase of class 1 integrons within anaerobic and aerobic digesters treating municipal wastewater solids
  publication-title: Environ. Sci. Technol.
– volume: 43
  start-page: 1523
  year: 1999
  end-page: 1524
  ident: b0070
  article-title: Nomenclature for new tetracycline resistance determinants
  publication-title: Antimicrob. Agents Chemother.
– volume: 66
  start-page: 370
  year: 2012
  end-page: 376
  ident: b0100
  article-title: Effect of Organic load and nutrient ratio on the operation stability of the moving bed bioreactor for kraft mill wastewater treatment and the incidence of polyhydroxyalkanoate biosynthesis
  publication-title: Water Sci. Technol.
– volume: 86
  start-page: 468
  year: 2012
  end-page: 476
  ident: b0125
  article-title: Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland)
  publication-title: Chemosphere
– volume: 447
  start-page: 345
  year: 2013
  end-page: 360
  ident: b0105
  article-title: Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review
  publication-title: Sci. Total Environ.
– volume: 43
  start-page: 3455
  year: 2009
  end-page: 3460
  ident: b0130
  article-title: Tetracycline resistance genes and tetracycline resistant lactose-fermenting
  publication-title: Environ. Sci. Technol.
– volume: 83
  start-page: 223
  year: 2010
  end-page: 233
  ident: b0085
  article-title: Methicillin resistant
  publication-title: Yale J. Biol. Med.
– volume: 91
  start-page: 1
  year: 2013
  end-page: 9
  ident: b0015
  article-title: Detection and fate of antibiotic resistant bacteria in wastewater treatment plants: a review
  publication-title: Ecotoxicol. Environ. Saf.
– volume: 36
  start-page: 429
  year: 1992
  end-page: 434
  ident: b0120
  article-title: Survey of methicillin resistant clinical strains of coagulase negative
  publication-title: Antimicrob. Agents Chemother.
– year: 2005
  ident: b0020
  article-title: Benson’s Microbiological Applications Laboratory Manual in General Microbiology
– volume: 4
  start-page: 1
  year: 2013
  end-page: 11
  ident: b0025
  article-title: Aerobic digestion reduces the quantity of antibiotic resistance genes in municipal wastewater solids
  publication-title: Front. Microbiol.
– volume: 6
  start-page: 519
  year: 2003
  end-page: 526
  ident: b0040
  article-title: Horizontal gene transfer emerging multidrug resistance in hospital bacteria
  publication-title: Acta Pharmacol.
– volume: 43
  start-page: 2823
  year: 1999
  end-page: 2830
  ident: b0110
  article-title: Nomenclature for macrolide and macrolide–lincosamide–streptogramin B resistance determinants
  publication-title: Antimicrob. Agents Chemother.
– volume: 46
  start-page: 350
  year: 2008
  end-page: 359
  ident: b0055
  article-title: Presence of methicillin resistant
  publication-title: J. Clin. Infectious Dis.
– reference: Louisiana Department of Wildlife and Fisheries, 2013. Statistics of commercial license sales from 1987–2011. <
– volume: 95
  start-page: 2
  year: 2014
  end-page: 10
  ident: b0045
  article-title: A survey of antibiotic – resistant bacteria in a sewage treatment plant in Thibodaux, Louisiana, USA
  publication-title: Int. Biodeterior. Biodegrad.
– volume: 46
  start-page: 7551
  year: 2012
  end-page: 7557
  ident: b0075
  article-title: Abundance and distribution of tetracycline resistance genes and mobile elements in an oxytetracycline production wastewater treatment system
  publication-title: Environ. Sci. Technol.
– year: 1998
  ident: b0005
  article-title: Standard Methods for the Analysis of Water and Wastewater
– volume: 45
  start-page: 9543
  year: 2011
  end-page: 9549
  ident: b0065
  article-title: Tertiary-treated municipal wastewater is a significant point source of antibiotic resistance genes into Duluth-superior harbor
  publication-title: Environ. Sci. Technol.
– volume: 10
  start-page: 559
  year: 2007
  end-page: 573
  ident: b0060
  article-title: Potential ecological and human health impacts of antibiotics and antibiotic-resistant bacteria from wastewater and treatment plants
  publication-title: J. Toxicol. Environ. Health
– volume: 525
  start-page: 397
  year: 2011
  end-page: 428
  ident: b0115
  article-title: Combating antimicrobial resistance: policy recommendations to save lives
  publication-title: Clin. Infectious Dis.
– reference: Center for Disease Control (CDC), 2014. Antibiotic resistance threats in the United States. <http//:CDC.gov> (accessed 27.10.14).
– volume: 87
  start-page: 1157
  year: 2010
  end-page: 1166
  ident: b0095
  article-title: Factors influencing antibiotic resistance burden in municipal wastewater treatment plants
  publication-title: Appl. Microbiol. Biotechnol.
– volume: 41
  start-page: 1143
  year: 2007
  end-page: 1151
  ident: b0010
  article-title: Tetracycline resistance genes in activated sludge wastewater treatment plants
  publication-title: Water Res.
– reference: > (accessed 11.11.14).
– volume: 76
  start-page: 4772
  year: 2010
  end-page: 4779
  ident: b0050
  article-title: Enumeration and characterization of antimicrobial resistant
  publication-title: Appl. Environ. Microbiol.
– volume: 66
  start-page: 370
  year: 2012
  ident: 10.1016/j.biortech.2015.01.052_b0100
  article-title: Effect of Organic load and nutrient ratio on the operation stability of the moving bed bioreactor for kraft mill wastewater treatment and the incidence of polyhydroxyalkanoate biosynthesis
  publication-title: Water Sci. Technol.
  doi: 10.2166/wst.2012.204
– volume: 10
  start-page: 559
  year: 2007
  ident: 10.1016/j.biortech.2015.01.052_b0060
  article-title: Potential ecological and human health impacts of antibiotics and antibiotic-resistant bacteria from wastewater and treatment plants
  publication-title: J. Toxicol. Environ. Health
  doi: 10.1080/15287390600975137
– volume: 41
  start-page: 1143
  year: 2007
  ident: 10.1016/j.biortech.2015.01.052_b0010
  article-title: Tetracycline resistance genes in activated sludge wastewater treatment plants
  publication-title: Water Res.
  doi: 10.1016/j.watres.2006.11.045
– volume: 43
  start-page: 1523
  year: 1999
  ident: 10.1016/j.biortech.2015.01.052_b0070
  article-title: Nomenclature for new tetracycline resistance determinants
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.43.6.1523
– volume: 6
  start-page: 519
  year: 2003
  ident: 10.1016/j.biortech.2015.01.052_b0040
  article-title: Horizontal gene transfer emerging multidrug resistance in hospital bacteria
  publication-title: Acta Pharmacol.
– year: 1998
  ident: 10.1016/j.biortech.2015.01.052_b0005
– volume: 45
  start-page: 681
  year: 2011
  ident: 10.1016/j.biortech.2015.01.052_b0090
  article-title: Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan
  publication-title: Water Res.
  doi: 10.1016/j.watres.2010.08.033
– volume: 4
  start-page: 1
  year: 2013
  ident: 10.1016/j.biortech.2015.01.052_b0025
  article-title: Aerobic digestion reduces the quantity of antibiotic resistance genes in municipal wastewater solids
  publication-title: Front. Microbiol.
  doi: 10.3389/fmicb.2013.00017
– volume: 87
  start-page: 1157
  year: 2010
  ident: 10.1016/j.biortech.2015.01.052_b0095
  article-title: Factors influencing antibiotic resistance burden in municipal wastewater treatment plants
  publication-title: Appl. Microbiol. Biotechnol.
  doi: 10.1007/s00253-010-2583-6
– year: 2005
  ident: 10.1016/j.biortech.2015.01.052_b0020
– volume: 36
  start-page: 429
  year: 1992
  ident: 10.1016/j.biortech.2015.01.052_b0120
  article-title: Survey of methicillin resistant clinical strains of coagulase negative Staphylococci for mecA gene distribution
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.36.2.429
– volume: 43
  start-page: 3455
  year: 2009
  ident: 10.1016/j.biortech.2015.01.052_b0130
  article-title: Tetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plants
  publication-title: Environ. Sci. Technol.
  doi: 10.1021/es803309m
– volume: 46
  start-page: 7551
  year: 2012
  ident: 10.1016/j.biortech.2015.01.052_b0075
  article-title: Abundance and distribution of tetracycline resistance genes and mobile elements in an oxytetracycline production wastewater treatment system
  publication-title: Environ. Sci. Technol.
  doi: 10.1021/es301145m
– volume: 91
  start-page: 1
  year: 2013
  ident: 10.1016/j.biortech.2015.01.052_b0015
  article-title: Detection and fate of antibiotic resistant bacteria in wastewater treatment plants: a review
  publication-title: Ecotoxicol. Environ. Saf.
  doi: 10.1016/j.ecoenv.2013.01.016
– ident: 10.1016/j.biortech.2015.01.052_b0030
– volume: 525
  start-page: 397
  year: 2011
  ident: 10.1016/j.biortech.2015.01.052_b0115
  article-title: Combating antimicrobial resistance: policy recommendations to save lives
  publication-title: Clin. Infectious Dis.
– volume: 44
  start-page: 9128
  year: 2010
  ident: 10.1016/j.biortech.2015.01.052_b0035
  article-title: Effect of temperature on the fate of genes encoding tetracycline resistance and the integrase of class 1 integrons within anaerobic and aerobic digesters treating municipal wastewater solids
  publication-title: Environ. Sci. Technol.
  doi: 10.1021/es102765a
– volume: 43
  start-page: 2823
  year: 1999
  ident: 10.1016/j.biortech.2015.01.052_b0110
  article-title: Nomenclature for macrolide and macrolide–lincosamide–streptogramin B resistance determinants
  publication-title: Antimicrob. Agents Chemother.
  doi: 10.1128/AAC.43.12.2823
– volume: 76
  start-page: 4772
  year: 2010
  ident: 10.1016/j.biortech.2015.01.052_b0050
  article-title: Enumeration and characterization of antimicrobial resistant Escherichia coli bacteria in effluent from municipal, hospital, and secondary treatment facility sources
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.02898-09
– volume: 447
  start-page: 345
  year: 2013
  ident: 10.1016/j.biortech.2015.01.052_b0105
  article-title: Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review
  publication-title: Sci. Total Environ.
  doi: 10.1016/j.scitotenv.2013.01.032
– volume: 46
  start-page: 350
  year: 2008
  ident: 10.1016/j.biortech.2015.01.052_b0055
  article-title: Presence of methicillin resistant Staphylococcus aureus in hospitals
  publication-title: J. Clin. Infectious Dis.
  doi: 10.1086/533591
– volume: 86
  start-page: 468
  year: 2012
  ident: 10.1016/j.biortech.2015.01.052_b0125
  article-title: Antibiotic resistant bacteria/genes dissemination in lacustrine sediments highly increased following cultural eutrophication of Lake Geneva (Switzerland)
  publication-title: Chemosphere
  doi: 10.1016/j.chemosphere.2011.09.048
– volume: 83
  start-page: 223
  year: 2010
  ident: 10.1016/j.biortech.2015.01.052_b0085
  article-title: Methicillin resistant Staphylococcus aureus: a pervasive pathogen highlights the need for antimicrobial development
  publication-title: Yale J. Biol. Med.
– ident: 10.1016/j.biortech.2015.01.052_b0080
– volume: 95
  start-page: 2
  year: 2014
  ident: 10.1016/j.biortech.2015.01.052_b0045
  article-title: A survey of antibiotic – resistant bacteria in a sewage treatment plant in Thibodaux, Louisiana, USA
  publication-title: Int. Biodeterior. Biodegrad.
  doi: 10.1016/j.ibiod.2014.05.028
– volume: 45
  start-page: 9543
  year: 2011
  ident: 10.1016/j.biortech.2015.01.052_b0065
  article-title: Tertiary-treated municipal wastewater is a significant point source of antibiotic resistance genes into Duluth-superior harbor
  publication-title: Environ. Sci. Technol.
  doi: 10.1021/es202775r
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Snippet •Antibiotic resistance genes in the raw and treated sewage were demonstrated.•mecA gene was present in both raw and treated sewage in all sampling...
Increasing uses and disposals of antibiotics to the environment have increased emergence of various antibiotic resistance. One of the sources for the spread of...
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SubjectTerms Anti-Bacterial Agents - chemistry
antibiotic resistance
Antibiotic resistance genes
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biological Oxygen Demand Analysis
Carbon - chemistry
DNA, Bacterial - chemistry
Drug Resistance, Bacterial - genetics
Erythromycin
Erythromycin - chemistry
genes
genetic transformation
Hydrogen-Ion Concentration
Louisiana
methicillin
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Microbial Sensitivity Tests
MRSA
Penicillin-Binding Proteins
Refuse Disposal - methods
RNA, Ribosomal, 16S - genetics
sewage
Sewage - microbiology
Sewage treatment
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Tetracycline
Tetracycline - chemistry
Waste Water - microbiology
wastewater treatment
Water Microbiology
Title Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA
URI https://dx.doi.org/10.1016/j.biortech.2015.01.052
https://www.ncbi.nlm.nih.gov/pubmed/25662190
https://www.proquest.com/docview/1680185922
https://www.proquest.com/docview/1836641467
Volume 188
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