Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 147; pp. 36 - 44
• Main Authors: Silva, Nuno H.C.S., Pinto, Ricardo J.B., Freire, Carmen S.R., Marrucho, Isabel M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2016
• Subjects: Animals; Biosensors; Chickens; Chlorides; Female; Fibrillation; Hydrogen-Ion Concentration; Kinetics; Lysozyme; Models, Molecular; Morphology; Muramidase - chemistry; Nanofibers; Nanofibers - chemistry; Nanostructure; Solvents; Solvents - chemistry; Tissue Engineering; Tissue Scaffolds

[Display omitted] •New and faster method for HEWL fibrillation using a deep eutectic solvent.•The conversion ratios of HEWL into protein nanofibers using a DES are higher than 90%.•Temperature has a key role in the acceleration of the fibrillation.•Temperature and pH significantly influence fibrils...