Determination of plasma cell labelling index with bromodeoxyuridine using a double immunoenzymatic technique

Myeloma plasma cells were double stained using peroxidase and alkaline phosphatase labelled monoclonal anti-BrdU and anti-intracytoplasmic immunoglobulins. Samples were methanol fixed; DNA was denatured with formamide. The results allowed easy identification of plasma cells, their cytological examin...

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Bibliographic Details
Published inStain technology Vol. 65; no. 1; p. 31
Main Authors Ffrench, M, Magaud, J P, Duhaut, P, Bryon, P A, Viala, J J
Format Journal Article
LanguageEnglish
Published United States 1990
Subjects
Bromodeoxyuridine
Cell Cycle
Humans
Immunoenzyme Techniques
Paraproteinemias - blood
Paraproteinemias - pathology
Plasma Cells - pathology
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Summary:Myeloma plasma cells were double stained using peroxidase and alkaline phosphatase labelled monoclonal anti-BrdU and anti-intracytoplasmic immunoglobulins. Samples were methanol fixed; DNA was denatured with formamide. The results allowed easy identification of plasma cells, their cytological examination and the calculation of percentage of plasma cells in S phase. Good correlation was found with the labelling index obtained with tritiated thymidine.
ISSN:0038-9153
DOI:10.3109/10520299009105605