Effect of age and exercise training on protein:protein interactions among eNOS and its regulatory proteins in rat aortas

• Published in: European journal of applied physiology Vol. 113; no. 11; pp. 2761 - 2768
• Main Authors: Luttrell, Meredith J., Seawright, John W., Wilson, Emily, Woodman, Christopher R.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2013; Springer Nature B.V
• Subjects: Acetylcholine - pharmacology; Aerobics; Age; Age Factors; Aging; Animals; Aorta - drug effects; Aorta - metabolism; Aorta - physiology; Bioavailability; Biomedical and Life Sciences; Biomedicine; Calmodulin - metabolism; Caveolin 1 - metabolism; Coronary vessels; Endothelium; Enzymes; Exercise; Fitness training programs; Heat shock proteins; Human Physiology; Hypotheses; Male; NG-Nitroarginine Methyl Ester - pharmacology; Nitric oxide; Nitric Oxide Synthase Type III - metabolism; Occupational Medicine/Industrial Medicine; Original Article; Physical Exertion; Physical fitness; Physiology; Protein Binding; Rats; Rats, Sprague-Dawley; Shear stress; Sports Medicine; Vasodilation; Veins & arteries; United States > US; Texas; Missouri; Caveolin-1; Co-immunoprecipitation; Fischer 344 rats; Calmodulin; Heat shock protein 90

Purpose We tested the hypothesis that impaired endothelium-dependent relaxation in aged aorta is due, in part, to altered protein:protein interactions between endothelial nitric oxide synthase (eNOS) and key regulatory proteins resulting in impaired nitric oxide (NO)-mediated relaxation. We also hyp...