PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus

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Published inApplied and Environmental Microbiology Vol. 80; no. 4; pp. 1477 - 1481
Main Authors Klevanskaa, Karina, Bier, Nadja, Stingl, Kerstin, Strauch, Eckhard, Hertwig, Stefan
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.02.2014
Subjects
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Cloning
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
E coli
Electroporation - methods
Escherichia coli
Escherichia coli - genetics
Gene Expression
Genes, Reporter
Genetic Vectors
Genomic Instability
Gram-negative bacteria
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Methods
Molecular Biology - methods
Molecular Sequence Data
Sequence Analysis, DNA
Transformation, Bacterial
Vibrio cholerae
Vibrio cholerae - genetics
Vibrio parahaemolyticus
Vibrio parahaemolyticus - genetics
Vibrio vulnificus
Vibrio vulnificus - genetics
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AbstractList An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10 6 transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae . To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli .
Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to AEM .asm.org, visit: AEM       
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 x 10^sup 6^ transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli. [PUBLICATION ABSTRACT]
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10(6) transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli.
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 106 transformants per mu g DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae. To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli.
ABSTRACT An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 × 10 6 transformants per μg DNA were achieved. The vector stably replicated in both V. vulnificus and Escherichia coli and was also successfully introduced into Vibrio parahaemolyticus and Vibrio cholerae . To demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (GFP) gene and the vvhBA hemolysin operon were inserted into the vector and functionally expressed in Vibrio and E. coli .
Author Stefan Hertwig
Eckhard Strauch
Karina Klevanskaa
Nadja Bier
Kerstin Stingl
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Snippet Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit...
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 ×...
ABSTRACT An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up...
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 x...
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 106...
An efficient electroporation procedure for Vibrio vulnificus was designed using the new cloning vector pVv3 (3,107 bp). Transformation efficiencies up to 2 ×...
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SubjectTerms Bacterial Proteins - analysis
Bacterial Proteins - genetics
Cloning
Deoxyribonucleic acid
DNA
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
E coli
Electroporation - methods
Escherichia coli
Escherichia coli - genetics
Gene Expression
Genes, Reporter
Genetic Vectors
Genomic Instability
Gram-negative bacteria
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Methods
Molecular Biology - methods
Molecular Sequence Data
Sequence Analysis, DNA
Transformation, Bacterial
Vibrio cholerae
Vibrio cholerae - genetics
Vibrio parahaemolyticus
Vibrio parahaemolyticus - genetics
Vibrio vulnificus
Vibrio vulnificus - genetics
Title PVv3, a New Shuttle Vector for Gene Expression in Vibrio vulnificus
URI http://aem.asm.org/content/80/4/1477.abstract
https://www.ncbi.nlm.nih.gov/pubmed/24362421
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