A Comparison of Assay Performance Measures in Screening Assays: Signal Window, Z′ Factor, and Assay Variability Ratio
In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and i...
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| Published in | Journal of biomolecular screening Vol. 11; no. 3; pp. 247 - 252 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.04.2006
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| Subjects | |
| Online Access | Get full text |
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| Abstract | In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays. |
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| AbstractList | In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays. |
| Author | Sittampalam, G. Sitta Iversen, Philip W. Eastwood, Brian J. Cox, Karen L. |
| Author_xml | – sequence: 1 givenname: Philip W. surname: Iversen fullname: Iversen, Philip W. – sequence: 2 givenname: Brian J. surname: Eastwood fullname: Eastwood, Brian J. – sequence: 3 givenname: G. Sitta surname: Sittampalam fullname: Sittampalam, G. Sitta – sequence: 4 givenname: Karen L. surname: Cox fullname: Cox, Karen L. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16490779$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/S0140-6736(86)90837-8 10.1177/108705719700200306 10.1177/108705710000500404 10.1021/cc9900706 10.1177/108705719900400206 10.1177/1087057105285611 |
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| References | Zhang (10.1177/1087057105285610_bib4) 2000; 2 Eastwood (10.1177/1087057105285610_bib5) 2006; 11 Sittampalam (10.1177/1087057105285610_bib1) 1997; 2 Taylor (10.1177/1087057105285610_bib3) 2000; 5 Bland (10.1177/1087057105285610_bib6) 1986; 1 Zhang (10.1177/1087057105285610_bib2) 1999; 4 |
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| SubjectTerms | Animals Cell Proliferation Lung - cytology Lung - drug effects Mink Models, Theoretical |
| Title | A Comparison of Assay Performance Measures in Screening Assays: Signal Window, Z′ Factor, and Assay Variability Ratio |
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